Best Prices Plant Hormone Indole-3-Acetic Acid Iaa
Nature
Indoleacetic asidhi inhengo yehupenyu.Zvigadzirwa zvakachena makristasi emashizha asina ruvara kana crystalline poda.Inoita rosy kana yaiswa pachiedza.Kunyungudika 165-166 ℃(168-170 ℃).Soluble mu anhydrous ethanol, ethyl acetate, dichloroethane, soluble mu ether uye acetone.Haina kunyungudika mubenzene, toluene, peturu uye chloroform.Isingasviki mumvura, mhinduro yayo ine aqueous inogona kusvibiswa ne ultraviolet chiedza, asi yakagadzikana kune chiedza chinooneka.Iyo sodium munyu uye potassium munyu yakagadzikana kupfuura iyo asidhi pachayo uye inonyungudika nyore mumvura.Zviri nyore decarboxylated kune 3-methylindole (skatine).Iine huviri hwekudyara kukura, uye zvikamu zvakasiyana zvechirimwa zvine manzwisisiro akasiyana kwairi, kazhinji mudzi wacho wakakura kupfuura bud rakakura kupfuura dzinde.Miti yakasiyana ine manzwiro akasiyana kwairi.
Kugadzirira nzira
3-indole acetonitrile inoumbwa nekuita kweindole, formaldehyde uye potassium cyanide pa150 ℃, 0.9 ~ 1MPa, uyezve hydrolyzed ne potassium hydroxide.Kana nekuita kweindole ne glycolic acid.Mune 3L Stainless steel autoclave, 270g (4.1mol) 85% potassium hydroxide, 351g (3mol) indole yakawedzerwa, uye ipapo 360g (3.3mol) 70% hydroxy acetic acid aqueous solution yakawedzerwa zvishoma nezvishoma.Yakavharwa kupisa kusvika ku250 ℃, ichikurudzira kwe18h.Kutonhora kusvika pasi pe50 ℃, wedzera 500ml mvura, uye kurunga pa 100 ℃ kwema 30min kunyungudutsa potassium indole-3-acetate.Kutonhorera kusvika 25 ℃, dururira autoclave zvinhu mumvura, uye wedzera mvura kusvika huwandu hwese hwave 3L.Iyo aqueous layer yakatorwa ne 500ml ethyl ether, acidified ne hydrochloric acid pa 20-30 ℃, uye yakanatswa ne indole-3-acetic acid.Sefa, geza mumvura inotonhora, omesa kure nechiedza, chigadzirwa 455-490g.
Biochemical kukosha
Property
Kuora zviri nyore muchiedza nemhepo, kwete kuchengetedza kwenguva refu.Yakachengeteka kuvanhu nemhuka.Soluble mumvura inopisa, ethanol, acetone, ether uye ethyl acetate, inonyungudika zvishoma mumvura, benzene, chloroform;Iyo yakagadzikana mualkaline solution uye inotanga kunyungudutswa mudiki shoma ye95% doro uye yozonyungudutswa mumvura kusvika pachiyero chakakodzera kana ichigadzirwa ne pure product crystallization.
Shandisa
Inoshandiswa sechirimwa chinosimudzira kukura uye analytical reagent.3-indole acetic acid uye zvimwe zvinhu zve auxin zvakadai se3-indole acetaldehyde, 3-indole acetonitrile uye ascorbic acid zviripo zvakasikwa muzvisikwa.Iyo inotangira 3-indole acetic acid biosynthesis muzvirimwa ndeye tryptophan.Basa rekutanga reauxin ndere kudzora kukura kwechirimwa, kwete chete kukurudzira kukura, asiwo kuvharidzira kukura uye kuvaka nhengo.Auxin haisipo chete mukusununguka mumasero emiti, asi inowanikwawo mune yakasungwa auxin iyo yakasungwa zvakasimba kune biopolymeric acid, nezvimwewo. Auxin inoumbawo conjugations nezvinhu zvakakosha, zvakadai se indole-acetyl asparagine, apentose indole-acetyl glucose, nezvimwewo. Iyi inogona kunge iri nzira yekuchengetedza yeauxin muchitokisi, uye zvakare nzira yekubvisa chepfu yekubvisa chepfu yeaxin yakawandisa.
Mhedzisiro
Plant auxin.Iyo inowanzozivikanwa yekukura kwehomoni muzvirimwa indoleacetic acid.Indoleacetic acid inogona kukurudzira kuumbwa kwepamusoro bud kuguma kwemiti yemiti, mashizha, mbesa, etc. Inotangira ndiyo tryptophan.Indoleacetic acid is achirimwa chekukura hormone.Somatin ine akawanda physiological mhedzisiro, ayo ane hukama nekuisa kwayo.Kudzikira kwakaderera kunogona kukurudzira kukura, kukwirisa kwakanyanya kunotadzisa kukura uye kunyange kuita kuti chirimwa chife, inhibition iyi ine chekuita nekuti inogona kukurudzira kuumbwa kwe ethylene.Iyo physiological mhedzisiro yeauxin inoratidzwa pamatanho maviri.Payero yemasero, auxin inogona kukurudzira cambium cell division;Kukurudzira bazi sero elongation uye inhibiting midzi masero kukura;Kurudzira xylem uye phloem cell kusiyanisa, kurudzira kugera bvudzi midzi uye gadzirisa callus morphogenesis.Panhengo uye padanho rechirimwa chose, auxin inoshanda kubva pambeu kusvika pakukura kwemuchero.Auxin controlled seedling mesocotyl elongation ine reversible red light inhibition;Apo iyo indoleacetic acid inotamirwa kudivi rezasi rebazi, bazi rinogadzira geotropism.Phototropism inoitika apo indoleacetic acid inotamirwa kune backlit side yematavi.Indoleacetic acid yakakonzera kutonga kwepamusoro.Kunonoka mashizha senescence;Auxin inoshandiswa kune mashizha inhibited abscission, nepo auxin ichiiswa kumagumo ekupedzisira kweabscission inosimudzira abscission.Auxin inokurudzira kutumbuka, inokurudzira parthenocarpy kukura, uye inonotsa kuibva kwemichero.
Shandisa
Indoleacetic acid ine yakakura spectrum uye yakawanda inoshandiswa, asi haiwanzo shandiswa nekuti iri nyore kusvibisa mukati nekubuda muzvirimwa.Mukutanga nhanho, yaishandiswa kukurudzira parthenocarpous uye michero-kugadzika kwemadomasi.Muchikamu chekutumbuka, maruva akanyoroveswa ne3000 mg/l mvura kuti agadzire michero isina mhodzi yemadomasi uye kunatsiridza mwero wekugadzika kwemichero.Imwe yekushandiswa kwekutanga yaive kusimudzira midzi yekucheka.Kunyura hwaro hwekucheka ne100 kusvika 1000 mg/l yemushonga wemushonga unogona kusimudzira kuumbwa kwemidzi yetii, gum tree, oak tree, metasequoia, mhiripiri nezvimwe zvirimwa, uye kukurumidza kukurumidza kubereka.1 ~ 10 mg/l indoleacetic acid uye 10 mg/L oxamyline yakashandiswa kukurudzira kudzura midzi yemadzinde emupunga.25 kusvika 400 mg / l yemvura spray chrysanthemum kamwe (mumaawa 9 e photoperiod), inogona kudzivisa kubuda kwemaruva emaruva, kunonoka maruva.Kukura muzuva refu kusvika 10 -5 mol/l concentration yakapfapfaidzwa kamwe chete, inogona kuwedzera maruva echikadzi.Kurapa mhodzi dzebhiti kunosimudzira kumera uye kunowedzera goho remudzi wechubhu uye shuga.
Nhanganyaya kune auxin
Nhanganyaya
Auxin (auxin) ikirasi ye endo native hormones ine isina unsaturated inonhuhwirira mhete uye acetic acid parutivi cheni, Chirungu chipfupiso IAA, iyo yakajairika yenyika, indole acetic acid (IAA).Muna 1934, Guo Ge et al.akazviona se indole acetic acid, saka itsika kugaro shandisa indole acetic acid serimwe izwi rekuti auxin.Auxin inogadzirwa mumashizha madiki akatambanudzwa uye apical meristem, uye inounganidzwa kubva kumusoro kusvika pahwaro nekutakura kureba kwephloem.Midzi inogadzirawo auxin, iyo inotakurwa kubva pasi kumusoro.Auxin muzvirimwa inoumbwa kubva tryptophan kuburikidza nenhevedzano yepakati.Nzira huru ndeye indoleacetaldehyde.Indole acetaldehyde inogona kuumbwa neiyo oxidation uye deamination ye tryptophan kuti indole pyruvate uye ipapo decarboxylated, kana inogona kuumbwa neiyo oxidation uye deamination ye tryptophan kune tryptamine.Iyo indole acetaldehyde inobva yaiswazve kune indole acetic acid.Imwe nzira inogoneka yekugadzira ndeye shanduko ye tryptophan kubva ku indole acetonitrile kuenda ku indole acetic acid.Indoleacetic acid inogona kuvharwa nekusunga ne aspartic acid kune indoleacetylaspartic acid, inositol kune indoleacetic acid kune inositol, glucose kune glucoside, uye mapuroteni kune indoleacetic acid-protein complex mumiti.Yakasungwa indoleacetic acid inowanzoita 50-90% yeindoleacetic acid mumiti, inogona kunge iri fomu yekuchengetedza yeauxin mumiti yemiti.Indoleacetic acid inogona kusvibiswa neoxidation ye indoleacetic acid, iyo inowanikwa mumiti yemiti.Auxins ane akawanda emuviri mhedzisiro, ayo ane hukama nekuisa kwavo.Kudzikira kwakaderera kunogona kukurudzira kukura, kukwirisa kwakanyanya kunotadzisa kukura uye kunyange kuita kuti chirimwa chife, inhibition iyi ine chekuita nekuti inogona kukurudzira kuumbwa kwe ethylene.Iyo physiological mhedzisiro yeauxin inoratidzwa pamatanho maviri.Payero yemasero, auxin inogona kukurudzira cambium cell division;Kukurudzira bazi sero elongation uye inhibiting midzi masero kukura;Kurudzira xylem uye phloem cell kusiyanisa, kurudzira kugera bvudzi midzi uye gadzirisa callus morphogenesis.Panhengo uye padanho rechirimwa chose, auxin inoshanda kubva pambeu kusvika pakukura kwemuchero.Auxin controlled seedling mesocotyl elongation ine reversible red light inhibition;Apo iyo indoleacetic acid inotamirwa kudivi rezasi rebazi, bazi rinogadzira geotropism.Phototropism inoitika apo indoleacetic acid inotamirwa kune backlit side yematavi.Indoleacetic acid yakakonzera kutonga kwepamusoro.Kunonoka mashizha senescence;Auxin inoshandiswa kune mashizha inhibited abscission, nepo auxin ichiiswa kumagumo ekupedzisira kweabscission inosimudzira abscission.Auxin inokurudzira kutumbuka, inokurudzira parthenocarpy kukura, uye inonotsa kuibva kwemichero.Mumwe munhu akauya nepfungwa yehomoni receptors.A hormone receptor ihombe molecular cell component inosungirirana chaizvo kune inoenderana hormone uye yobva yatanga nhevedzano yemaitiro.Iyo yakaoma ye indoleacetic acid uye receptor ine mhedzisiro miviri: yekutanga, inoshanda pa membrane mapuroteni, inokanganisa yepakati acidification, ion pombi yekufambisa uye tension shanduko, inova inokurumidza kuita.< maminitsi gumi);Chechipiri ndechekuita nucleic acids, zvichiita kuti sero rusvingo kuchinja uye protein synthesis, iyo inononoka kuita (10 maminitsi).Medium acidification chinhu chakakosha chekukura kwesero.Indoleacetic acid inogona kumisa iyo ATP (adenosine triphosphate) enzyme pa plasma membrane, inokurudzira hydrogen ions kuti ibude musero, kuderedza pH kukosha kwepakati, kuitira kuti enzyme iite, hydrolyze iyo polysaccharide yemadziro emadziro, saka. kuti rusvingo rwesero rwakapfava uye sero rinowedzerwa.Kutungamira kweindoleacetic acid kwakakonzera kutaridzika kweiyo chaiyo nhume RNA (mRNA) kutevedzana, iyo yakachinja mapuroteni synthesis.Indoleacetic acid kurapwa kwakashandurawo kunyungudika kwesero madziro, zvichibvumira kukura kwesero kuenderera.Iko kukura kwekusimudzira maitiro eauxin inonyanya kusimudzira kukura kwemaseru, kunyanya kurebesa kwemasero, uye haina mhedzisiro pakupatsanurwa kwesero.Chikamu chechirimwa chinonzwa kukurudzirwa kwechiedza chiri pamucheto wedzinde, asi chikamu chekukotama chiri pazasi pepipo, imhaka yekuti masero ari pazasi pepipu ari kukura nekuwedzera, uye ndiwo anonyanya kunzwa. nguva ye auxin, saka iyo auxin ine simba guru pakukura kwayo.Kuchembera tishu kukura hormone haishande.Chikonzero nei auxin ichigona kusimudzira kukura kwemichero uye kudzika midzi yekucheka ndeyekuti auxin inogona kushandura kugoverwa kwezvinovaka muviri muchirimwa, uye zvimwe zvinovaka muviri zvinowanikwa muchikamu nekupfuma auxin kugovera, kugadzira nzvimbo yekugovera.Auxin inogona kukonzeresa kuumbwa kwemadomasi asina mhodzi nekuti mushure mekurapa mabuds asina kuviriswa neauxin, ovary yetomato bud inova nzvimbo yekuparadzira yezvinovaka muviri, uye chikafu chinogadzirwa nephotosynthesis yemashizha chinoramba chichiendeswa kune ovary, uye ovary inokura. .
Chizvarwa, chekufambisa uye kugovera
Izvo zvikamu zvikuru zve auxin synthesis ndeye meristant tissues, kunyanya madiki mabuds, mashizha, uye kukura mbeu.Auxin inogoverwa munhengo dzese dzemuviri wechirimwa, asi yakanyatso kudzika muzvikamu zvekukura kwakasimba, senge coleopedia, buds, midzi apex meristem, cambium, kukura mbeu nemichero.Pane nzira nhatu dzekutakura auxin muzvirimwa: lateral transport, polar transport uye non-polar transport.Lateral transport (backlight transport yeauxin mumuromo wecoleoptile inokonzerwa neunilateral light, padhuze-ivhu divi kutakurwa kweauxin mumidzi uye madzinde ezvirimwa painochinjika).Polar transport (kubva kumucheto wepamusoro we morphology kusvika kumucheto wepasi we morphology).Non-polar kutakura (mumatishu akakura, auxin inogona kunge isiri-polar kutakurwa kuburikidza nephloem).
Hunyambiri hwekuita kwemuviri
Kudzika kwakadzika kunokurudzira kukura, kukwidzika kwepamusoro kunodzivisa kukura.Mitezo yemiti yakasiyana ine zvinodiwa zvakasiyana kune yakakwana yekusangana kweauxin.The optimum concentration yaive ye10E-10mol/L yemidzi, 10E-8mol/L yemabuds uye 10E-5mol/L yemadzinde.Auxin analogues (yakadai se naphthalene acetic acid, 2, 4-D, nezvimwewo) inowanzoshandiswa mukugadzira kugadzirisa kukura kwemiti.Semuyenzaniso, panogadzirwa bhinzi, tsinga inokodzera kumera ndiyo inoshandiswa kurapa bhinzi.Somugumisiro, midzi uye buds zvinodziviswa, uye mashizha anogadzirwa kubva ku hypocotyl akanyatsogadzirwa.Iyo apex mukana wekukura kwechirimwa chinotarirwa nehunhu hwekutakura hwezvirimwa zve auxin uye huviri hwe auxin physiological mhedzisiro.Iyo apex bud yechirimwa hunde ndiyo inonyanya kushanda yekugadzira auxin, asi iyo inogadzirwa auxin inogadzirwa paapex bud inogara ichiendeswa kune hunde kuburikidza nekufambisa kunoshanda, saka kuwanda kweauxin muapex bud pachayo hakuna kukwirira. ukuwo kuwanda kwehunde diki kwakakwira.Iyo inonyanya kukodzera pakukura kwe stem, asi ine inhibitory mhedzisiro pabuds.Iyo yakakwirira yekusangana kwe auxin munzvimbo iri padyo nepamusoro bud, iyo inosimba inhibitory mhedzisiro padivi bud, ndosaka zvirimwa zvakawanda zvirefu zvinogadzira pagoda chimiro.Nekudaro, hazvisi zvese zvinomera zvine hutongi hwakasimba hwepamusoro, uye mamwe makwenzi anotanga kushatisa kana kutoderera mushure mekugadzirwa kweapex bud kwenguva yakati rebei, achirasikirwa nehutongi hwekutanga hwepamusoro, saka chimiro chemuti che shrub hachisi pagoda. .Nekuti yakanyanya kuwanda kweauxin ine mhedzisiro yekuvharisa kukura kwechirimwa, kugadzirwa kweiyo yakakwira yeauxin analogues inogona kushandiswa semishonga yesora, kunyanya kune dicotyledonous sora.
Auxin analogues: NAA, 2, 4-D.Nekuti auxin iripo mune zvidiki muzvirimwa, uye hazvisi nyore kuchengetedza.Kuti zvigadzirise kukura kwezvirimwa, kuburikidza nemakemikari synthesis, vanhu vakawana auxin analogues, ane mhedzisiro yakafanana uye anogona kugadzirwa-zhinji, uye akashandiswa zvakanyanya mukugadzirwa kwekurima.Mhedzisiro yegiravhiti yepasi pakugovera auxin: kukura kwemashure uye kukura kwemidzi kunokonzerwa negiravhiti repasi, chikonzero ndechekuti giravhiti repasi rinokonzera kusaenzana kugovaniswa kweauxin, iyo inonyanya kugoverwa mudivi repedyo. iyo hunde uye zvishoma yakagoverwa mudivi rekuseri.Nekuda kwekuti huwandu hwakanyanya hwe auxin muhunde hwakanga hwakakwirisa, auxin yakawanda mudivi redzinde yaisimudzira, saka divi repedyo redzinde raikura nekukurumidza kupfuura kuseri kwedzinde, uye raichengeta kukwira kumusoro kwedzinde.Kune midzi, nekuti iyo yakakwana yekuisa auxin mumidzi yakadzikira, yakawanda auxin padyo nedivi repasi ine inhibitory mhedzisiro pakukura kwemasero emidzi, saka kukura kwepedyo nedivi repasi kunononoka kupfuura iyo yekuseri side, uye iyo geotropic kukura kwemidzi inochengetwa.Pasina simba rinokwevera zvinhu pasi, midzi hairegi ichidzika pasi.Mhedzisiro yekusarema pakukura kwechirimwa: midzi inokura yakananga pasi uye kukura kwedzinde kure nevhu kunokonzerwa negiravhiti repasi, iro rinokonzerwa nekugovaniswa kusina kuenzana kweauxin pasi pekupinzwa kwegiravhiti repasi.Munzvimbo isina uremu yenzvimbo, nekuda kwekurasikirwa kwesimba, kukura kwetsinde kucharasikirwa nekudzokera shure, uye midzi icharasikirwawo nemaitiro ekukura kwevhu.Nekudaro, iyo yepamusoro mukana wekukura kwe stem ichiripo, uye polar kutakura kwe auxin hakukanganiswe negiravhiti.
