Kurima ndicho chinhu chakakosha zvikuru mumisika yepasi rose, uye masisitimu ezvakatipoteredza anotarisana nematambudziko akawanda. Kushandiswa kwefetereza yemakemikari pasi rose kuri kukura uye kunoita basa guru mukukohwa kwezvirimwa1. Zvisinei, zvirimwa zvinorimwa nenzira iyi hazvina nguva yakakwana yekukura nekukura zvakanaka saka hazviwani hunhu hwakanaka hwezvirimwa2. Pamusoro pezvo, mishonga ine chepfu inogona kuungana mumuviri wemunhu nevhu3. Nokudaro, pane chikonzero chekugadzira mhinduro dzinochengetedza nharaunda uye dzinogara kwenguva refu kuti kuderedza kudiwa kwefetereza yemakemikari. Zvipembenene zvinobatsira zvinogona kuva sosi yakakosha yemakemikari echisikigo anoshanda muhupenyu4.
Mapoka e endophytic mumashizha anosiyana zvichienderana nerudzi rwemiti inogarwa kana genotype, danho rekukura kwemiti, uye chimiro chemiti. 13 Ongororo dzakawanda dzakaratidza kuti Azospirillum, Bacillus, Azotobacter, Pseudomonas, uye Enterobacter vane mukana wekutikukurudzira kukura kwezvirimwa. 14 Uyezve, Bacillus neAzospirillum ndidzo mbesa dzePGPB dzakanyatso tsvakwa zvakanyanya maererano nekuvandudza kukura negoho rezvirimwa. 15 Zvidzidzo zvakaratidza kuti kubaiwa pamwe chete kweAzospirillum brasiliensis neBradyrhizobium mumbesa kunogona kuwedzera goho rezviyo, gorosi, soya, nebhinzi. 16, 17 Zvidzidzo zvakaratidza kuti kubaiwa kweSalicornia neBacillus licheniformis nedzimwe PGPB zvinokurudzira kukura kwezvirimwa uye kutora zvinovaka muviri. 18 Azospirillum brasiliensis Sp7 neBacillus sphaericus UPMB10 zvinovandudza kukura kwemidzi yebhanana rinotapira. Saizvozvowo, mbeu dzefennel dzakaoma kurima nekuda kwekukura kusina kunaka kwezvirimwa uye kumera kushoma, kunyanya kana kusanaya kwemvura. Kurapa mbeu nePseudomonas fluorescens neTrichoderma harzianum kunovandudza kukura kwekutanga kwembesa dzefennel kana kusanaya kwemvura. Kune stevia, zvidzidzo zvakaitwa kuti zviongorore mhedzisiro ye mycorrhizal fungi uye kukura kwezvirimwa zvinokurudzira rhizobacteria (PGPR) pakukwanisa kwezvisikwa kukura, kuunganidza metabolites yechipiri, uye kuratidza majini anobatanidzwa mu biosynthesis. Sekureva kwaRahi et al.22, kubaiwa kwezvirimwa zvine PGPR dzakasiyana kwakavandudza kukura kwazvo, photosynthetic index, uye kuunganidzwa kwestevioside nestevioside A. Kune rumwe rutivi, kubaiwa kwestevia ne rhizobia inokurudzira kukura kwezvirimwa uye arbuscular mycorrhizal fungi kwakakurudzira kureba kwezvirimwa, stevioside, mineral, uye pigment contents.23 Oviedo-Pereira et al.24 vakashuma kuti endophytes dzinotsamwisa Enterobacter hormaechei H2A3 neH5A2 dzakawedzera huwandu hweSG, dzakakurudzira huwandu hwetrichome mumashizha, uye dzakakurudzira kuunganidzwa kwemetabolites chaiyo mutrichomes, asi hadzina kukurudzira kukura kwezvirimwa;
GA3 ndeimwe yemapuroteni akakosha uye anoshanda muhupenyu hwese akaita segibberellin31. Kurapa stevia neGA3 kunze kunogona kuwedzera kureba kwehunde uye kutumbuka32. Kune rumwe rutivi, zvimwe zvidzidzo zvakashuma kuti GA3 inokurudzira zvirimwa kuti zvigadzire metabolites dzechipiri dzakadai sema antioxidants ne pigments, uye zvakare inzira yekudzivirira33.
Hukama hwe phylogenetic hwe isolates maererano nedzimwe mhando dze strain. Nhamba dzekusvika kweGenBank dzakapihwa mumabhureki.
Mabasa eAmylase, cellulase uye protease anoratidzwa semabhandi akajeka akatenderedza makoroni, nepo machena akatenderedza makoroni achiratidza mashandiro e lipase. Sezvakaratidzwa muTafura 2, B. paramycoides SrAM4 inogona kugadzira mahydrolase ese, nepo B. paralicheniformis SrMA3 inogona kugadzira ma enzyme ese kunze kwe cellulase, uye B. licheniformis SrAM2 inogadzira cellulase chete.
Mabhakitiriya akawanda akakosha akabatanidzwa nekuwedzera kwekugadzirwa kwemetabolite yechipiri mumiti yemishonga uye inonhuhwirira74. Ma antioxidants ese e enzyme uye asiri enzyme akawedzera zvakanyanya muS. rebaudiana Shou-2 zvichienzaniswa nekudzora. Mhedzisiro yakanaka yePGPB paTPC mumupunga yakashumwawo naChamam et al.75; Uyezve, mhedzisiro yedu inoenderana nemhedzisiro yeTPC, TFC, uye DPPH muS. rebaudiana, iyo yakakonzerwa nekushanda kwakabatana kwePiriformospora indica naAzotobacter chroococcum76. TPC neTFC77 zvaive zvakanyanya mumiti yebasil yakarapwa nehutachiona zvichienzaniswa nemiti isina kurapwa. Uyezve, kuwedzera kwe antioxidants kunogona kuitika nekuda kwezvikonzero zviviri: ma enzymes e hydrolytic anokurudzira nzira dzekudzivirira zvirimwa nenzira imwecheteyo nehutachiona hwezvirwere kusvika chirimwa chajaira kugara mumabhakitiriya78. Chechipiri, PGPB inogona kushanda semutangi wekugadzirwa kwemakemikari anoshanda akaumbwa kuburikidza nenzira yeshikimate mumiti yakakwirira nehutachiona 79.
Zvakabuda zvakaratidza kuti paive nehukama hwakabatana pakati pehuwandu hwemashizha, kuratidzwa kwemajini uye kugadzirwa kweSG apo marudzi akawanda aibaiwa pamwe chete. Kune rumwe rutivi, kubaiwa kaviri kwaive kwakanaka kupfuura kubaiwa kamwe chete maererano nekukura kwezvirimwa uye kubereka.
Ma enzyme ehydrolytic akaonekwa mushure mekubaya mabhakitiriya pa agar medium ine indicator substrate uye kuisirwa mudumbu pa 28°C kwemazuva 2-5. Mushure mekuputira mabhakitiriya pa starch agar medium, amylase activity yakaongororwa uchishandisa iodine 100 solution. Activity ye cellulase yakaongororwa uchishandisa 0.2% aqueous Congo red reagent zvichienderana nenzira yaKianngam et al. 101. Activity yeprotease yakaonekwa kuburikidza nenzvimbo dzakajeka dzakatenderedza makoroni akaiswa pa skim milk agar medium sezvakatsanangurwa naCui et al. 102. Kune rumwe rutivi, lipase 100 yakaonekwa mushure mekubayiwa pa Tween agar medium.
Nguva yekutumira: Ndira-06-2025