Kupararira kwemvura muGuizhou Province mwaka wega wega hakuna kuenzana, nemvura yakawanda muchirimo nemuzhizha, asi mbesa dzerapeseed dzinogona kubatwa nekusanaya kwemvura mumatsutso nemuchando, izvo zvinokanganisa goho zvakanyanya. Mustard chirimwa chakakosha chembeu yemafuta chinonyanya kurimwa muGuizhou Province. Chine simba guru rekusanaya kwemvura uye chinogona kurimwa munzvimbo dzine makomo. Chitubu chakapfuma chemajini asinganeti kusanaya kwemvura. Kuwanikwa kwemajini asinganeti kusanaya kwemvura kwakakosha zvikuru pakuvandudza mhando dzemasitadhi. uye hunyanzvi muzviwanikwa zve germplasm. Mhuri yeGRF inoita basa rakakosha mukukura nekukura kwezvirimwa uye mhinduro yekusanaya kwemvura. Parizvino, majini eGRF akawanikwa muArabidopsis 2, mupunga (Oryza sativa) 12, rapeseed 13, donje (Gossypium hirsutum) 14, gorosi (Triticum). aestivum)15, pearl millet (Setaria italica)16 uye Brassica17, asi hapana mishumo yemajini eGRF akawanikwa mumasitadhi. Muchidzidzo ichi, majini emhuri yemustard eGRF akaonekwa padanho remajini ese uye hunhu hwawo hwemuviri nemakemikari, hukama hwekushanduka-shanduka, homology, conserved motifs, gene structure, gene duplications, cis-elements uye seedling stage (danho remashizha mana) zvakaongororwa. Maitiro ekutaura pasi pekuoma kwemvura akaongororwa zvakakwana kuti ape hwaro hwesainzi hwezvidzidzo zvakawanda pamusoro pekushanda kwemajini eBjGRF mukupindura kusanaya kwemvura uye kupa majini akakodzera ekuberekesa mustard inotsungirira kusanaya kwemvura.
Majini makumi matatu nemana eBjGRF akawanikwa muBrassica juncea genome achishandisa kutsvaga kaviri kweHMMER, ese ane maQLQ neWRC domains. MaCDS sequences emajini eBjGRF akaonekwa anoratidzwa muSupplementary Table S1. BjGRF01–BjGRF34 akapihwa mazita zvichienderana nenzvimbo yawo pachromosome. Hunhu hwefizikisi hwemhuri iyi hunoratidza kuti urefu hweamino acid hunosiyana zvakanyanya, kubva pa261 aa (BjGRF19) kusvika 905 aa (BjGRF28). Poindi yeisoelectric yeBjGRF inotangira pa6.19 (BjGRF02) kusvika 9.35 (BjGRF03) neavhareji ye8.33, uye 88.24% yeBjGRF ipuroteni yekutanga. Huremu hweBjGRF hwakafanotaurwa hunobva pa29.82 kDa (BjGRF19) kusvika pa102.90 kDa (BjGRF28); indekisi yekusagadzikana kwemapuroteni eBjGRF inotangira pa51.13 (BjGRF08) kusvika pa78.24 (BjGRF19), ese anopfuura 40, zvichiratidza kuti indekisi yefatty acid inotangira pa43.65 (BjGRF01) kusvika pa78.78 (BjGRF22), avhareji yehydrophilicity (GRAVY) inotangira pa -1.07 (BjGRF31) kusvika pa -0.45 (BjGRF22), mapuroteni ese ehydrophilic eBjGRF ane GRAVY isina kunaka, izvo zvinogona kunge zvichikonzerwa nekushaikwa kwehydrophobicity inokonzerwa nemasara. Kufanotaura kwenzvimbo iri pasi pemasero kwakaratidza kuti mapuroteni makumi matatu nerimwe eBjGRF anogona kuwanikwa munucleus, BjGRF04 inogona kuwanikwa muperoxisomes, BjGRF25 inogona kuwanikwa mucytoplasm, uye BjGRF28 inogona kuwanikwa muchloroplasts (Tafura 1), zvichiratidza kuti maBjGRF anogona kuwanikwa munucleus uye anoita basa rakakosha rekutonga sechikamu chekunyora.
Kuongororwa kwePhylogenetic yemhuri dzeGRF mumarudzi akasiyana kunogona kubatsira kudzidza mashandiro emajini. Nokudaro, ma amino acid akazara e35 rapeseed, 16 turnip, 12 mupunga, 10 millet uye 9 Arabidopsis GRFs akatorwa uye muti wephylogenetic wakagadzirwa zvichibva pamajini eBjGRF makumi matatu nemana akaonekwa (Mufananidzo 1). Mhuri nhatu idzi dzine nhamba dzakasiyana dzenhengo; 116 GRF TFs dzakakamurwa kuita mhuri nhatu dzakasiyana (mapoka A~C), dzine 59 (50.86%), 34 (29.31%) uye 23 (19.83)% yemaGRF, zvichiteerana. Pakati pavo, nhengo dzemhuri dzeBjGRF makumi matatu nemana dzakapararira mumhuri nhatu: nhengo gumi nenhatu muboka A (38.24%), nhengo gumi nembiri muboka B (35.29%) uye nhengo pfumbamwe muboka C (26.47%). Mukuita kwe polyploidization yemasitadhi, huwandu hwemajini eBjGRF mumhuri dzakasiyana dzakasiyana hwakasiyana, uye kuwedzera nekurasikirwa kwemajini kungave kwakaitika. Zvakakosha kuziva kuti hapana kugoverwa kweGRF dzemupunga nedzemupunga muboka C, nepo paine maGRF maviri emupunga neGRF imwe yemupunga muboka B, uye mazhinji emaGRF emupunga nedzemupunga akarongwa muboka rimwe chete, zvichiratidza kuti maBjGRF ane hukama hwepedyo nemadicots. Pakati pawo, zvidzidzo zvakadzama pamusoro pebasa reGRF muArabidopsis thaliana zvinopa hwaro hwezvidzidzo zvinoshanda zveBjGRF.
Muti wemasitadhi une phylogenetic tree unosanganisira Brassica napus, Brassica napus, mupunga, mapfunde uye nhengo dzemhuri yeArabidopsis thaliana GRF.
Kuongororwa kwemajini anodzokororwa mumhuri yemasitadhi GRF. Mutsetse wegrey uri kumashure unomiririra bhuroko rakaenderana mugenome yemasitadhi, mutsetse mutsvuku unomiririra kudzokororwa kwejini reBjGRF kwakapatsanurwa;
Kuonekwa kwemajini eBjGRF pasi pekusanaya kwemvura padanho rechina remashizha. Data reqRT-PCR rinoratidzwa muSupplementary Table S5. Musiyano mukuru mudata unoratidzwa nemavara madiki.
Sezvo mamiriro ekunze epasi rose achiramba achichinja, kudzidza kuti zvirimwa zvinorarama sei nekushaikwa kwemvura uye kuvandudza nzira dzazvo dzekushivirira kwave nyaya inonyanya kutsvakurudzwa18. Mushure mekushaikwa kwemvura, chimiro chechimiro, kuratidzwa kwemajini uye maitiro ekugadzirisa metabolic ezvirimwa zvichachinja, izvo zvinogona kutungamira kukumiswa kwe photosynthesis uye kukanganiswa kwe metabolic, zvichikanganisa goho uye mhando yezvirimwa19,20,21. Kana zvirimwa zvikanzwa zviratidzo zvekushaikwa kwemvura, zvinogadzira vatumwa vechipiri vakaita seCa2+ nephosphatidylinositol, zvinowedzera huwandu hwecalcium ion mukati memasero uye zvinomutsa network yekutonga yeprotein phosphorylation pathway22,23. Puroteni yekupedzisira inobatanidzwa zvakananga mukudzivirira kwemasero kana kudzora kuratidzwa kwemajini ane hukama nekushaikwa kwemvura kuburikidza neTFs, zvichiwedzera kushivirira kwezvirimwa kustress24,25. Saka, TFs dzinoita basa rakakosha mukupindura kushaikwa kwemvura. Zvichienderana ne sequence uye DNA binding properties ye drought stress responsive TFs, TFs dzinogona kukamurwa mumhuri dzakasiyana, dzakadai seGRF, ERF, MYB, WRKY nedzimwe mhuri26.
Mhuri yemajini eGRF imhando yeTF yezvirimwa ine mabasa akakosha muzvinhu zvakasiyana-siyana zvakaita sekukura, kukura, kufambiswa kwemashoko uye mhinduro dzekudzivirira zvirimwa27. Kubva pakawanikwa jini rekutanga reGRF muO. sativa28, majini akawanda eGRF akaonekwa mumarudzi akawanda uye akaratidzwa kuti anokanganisa kukura kwezvirimwa, kukura uye kushushikana8, 29, 30,31,32. Nekuburitswa kweBrassica juncea genome sequence, kuzivikanwa kwemhuri yemajini eBjGRF kwakakwanisika33. Muchidzidzo ichi, majini eBjGRF makumi matatu nemana akaonekwa mugenome yese yemasitadhi uye akatumidzwa zita rekuti BjGRF01–BjGRF34 zvichibva panzvimbo yavo yemakromosomal. Ose ane maQLQ neWRC domains akachengetedzwa zvakanyanya. Kuongororwa kwehunhu hwefizikisiki kwakaratidza kuti mutsauko munhamba dzeamino acid uye huremu hwemamorekuru emapuroteni eBjGRF (kunze kweBjGRF28) hauna kukosha, zvichiratidza kuti nhengo dzemhuri yeBjGRF dzinogona kunge dziine mabasa akafanana. Kuongororwa kwechimiro chemajini kwakaratidza kuti 64.7% yemajini eBjGRF aive nemaexons mana, zvichiratidza kuti chimiro chemajini eBjGRF chakachengetedzwa zvakanyanya mukushanduka-shanduka, asi huwandu hwemaexons mumajini eBjGRF10, BjGRF16, BjGRP28 uye BjGRF29 hwakakura. Zvidzidzo zvakaratidza kuti kuwedzerwa kana kubviswa kwemaexons kana ma intron kunogona kutungamira mumisiyano muchimiro chemajini uye mashandiro, nokudaro kugadzira majini matsva34,35,36. Saka, tinofungidzira kuti intron yeBjGRF yakarasika panguva yekushanduka-shanduka, izvo zvinogona kukonzera shanduko mukushanda kwemajini. Zvichienderana nezvidzidzo zviripo, takaonawo kuti huwandu hwema intron hwakabatana nekutaura kwemajini. Kana huwandu hwema introns mujini hwakakura, jini rinogona kukurumidza kupindura kune zvinhu zvakasiyana-siyana zvisina kunaka.
Kudzokorora majini chinhu chikuru mukushanduka kwemajini uye majini37. Zvidzidzo zvakabatana zvakaratidza kuti kudzokororwa kwemajini hakungowedzeri huwandu hwemajini eGRF chete, asiwo kunoshanda senzira yekugadzira majini matsva kubatsira zvirimwa kuti zvijairane nemamiriro akasiyana-siyana ezvakatipoteredza asina kunaka38. Huwandu hwemajini maviri akapetwa kaviri akawanikwa muchidzidzo ichi, ese ari maviri akapetwa kaviri, zvichiratidza kuti kudzokororwa kwezvikamu ndiyo nzira huru yekuwedzera huwandu hwemajini mumhuri iyi. Zvakataurwa mumabhuku kuti kudzokororwa kwezvikamu kunogona kukurudzira kukura kwenhengo dzemhuri yemajini eGRF muArabidopsis nestrawberry, uye hapana kudzokororwa kwemhuri yemajini aya kwakawanikwa mune chero rudzi27,39. Mhedzisiro yechidzidzo ichi inoenderana nezvidzidzo zviripo pamhuri dzeArabidopsis thaliana nestrawberry, zvichiratidza kuti mhuri yeGRF inogona kuwedzera huwandu hwemajini uye kugadzira majini matsva kuburikidza nekudzokororwa kwezvikamu mumiti yakasiyana.
Muchidzidzo ichi, majini eBjGRF anosvika 34 akaonekwa mumasitadhi, ayo akakamurwa kuita mhuri nhatu. Majini aya akaratidza maitiro akafanana uye maumbirwo emajini. Kuongorora kweCollinearity kwakaratidza mapeya makumi mana nemasere ezvikamu zvakafanana mumasitadhi. Dunhu reBjGRF promoter rine zvinhu zvinoshanda zvecis zvine chekuita nekupindura kwechiedza, mhinduro yemahormone, mhinduro yekumanikidzwa kwezvakatipoteredza, uye kukura nekukura. Kuratidzwa kwemajini eBjGRF anosvika 34 kwakaonekwa padanho rekukura kwemasitadhi (midzi, madzinde, mashizha), uye patani yekutaura kwemajini gumi eBjGRF pasi pemamiriro ekusanaya kwemvura. Zvakawanikwa kuti mapatani ekutaura kwemajini eBjGRF pasi pemamiriro ekusanaya kwemvura akafanana uye anogona kunge akafanana. kubatanidzwa mukusanaya kwemvura. Majini eBjGRF03 neBjGRF32 anogona kutamba mabasa akanaka ekudzora mukusanaya kwemvura, nepo majini eBjGRF06 neBjGRF23 achitamba mabasa mukusanaya kwemvura semajini anotarisirwa emiR396. Zvose, chidzidzo chedu chinopa hwaro hwezvinhu zvipenyu zvekuwanikwa kwebasa remajini eBjGRF mune ramangwana muzvirimwa zveBrassicaceae.
Mbeu dzemasitadhi dzakashandiswa mukuyedza uku dzakapihwa neGuizhou Oil Seed Research Institute, Guizhou Academy of Agricultural Sciences. Sarudzai mbeu dzese modzidyara muvhu (substrate: ivhu = 3:1), mounganidza midzi, madzinde nemashizha mushure mechikamu chemashizha mana. Zvirimwa zvakarapwa ne20% PEG 6000 kuti zvitevedzere kusanaya kwemvura, uye mashizha akatorwa mushure memaawa 0, 3, 6, 12 uye 24. Mienzaniso yese yezvirimwa yakabva yangoomeswa mu liquid nitrogen yozochengetwa mu -80°C firiji yekuedza kunotevera.
Ruzivo rwese rwakawanikwa kana kuongororwa panguva yekudzidza uku rwunowanikwa muchinyorwa chakabudiswa uye mumafaira eruzivo rwekuwedzera.
Nguva yekutumira: Ndira-22-2025