Kugoverwa kwemwaka wekunaya kwemvura muGuizhou Province hakuna kuenzana, kuine kunaya kwakawanda muchirimo nechirimo, asi mbesa dzembeu dzinobatwa nedambudziko rekusanaya kwemvura mumatsutso nemuchando, izvo zvinokanganisa goho. Mustard chirimwa cheoiri chakakosha chinorimwa mudunhu reGuizhou. Iine simba rekutsungirira kusanaya kwemvura uye inogona kurimwa munzvimbo dzine makomo. Ipfuma yakawanda yemajini anoramba kusanaya kwemvura. Kuwanikwa kwemajini anoramba kusanaya kwemvura kwakakosha zvakanyanya pakuvandudzwa kwemhando yemasitadhi. uye innovation mune germplasm zviwanikwa. Mhuri yeGRF inoita basa rakakosha mukukura nekukura kwechirimwa uye nemhinduro yekusanaya kwemvura. Parizvino, majini eGRF akawanikwa muArabidopsis 2, mupunga (Oryza sativa) 12, rapeseed 13, donje (Gossypium hirsutum) 14, gorosi (Triticum). aestivum)15, pearl millet (Setaria italica)16 neBrassica17, asi hapana mishumo yemajini eGRF akaonekwa mumasitadhi. Muchidzidzo ichi, GRF mhuri yemajini yemasitadhi yakaonekwa pajenome-yakakura pamwero uye maitiro avo emuviri nemakemikari, hukama hwekushanduka-shanduka, homology, kuchengetedza motifs, gene structure, gene duplications, cis-elements uye seedling stage (mana-shizha nhanho) yakaongororwa. Matauriro emaitiro pasi pekusanaya kwemvura akaongororwa zvakadzama kuti ape hwaro hwesainzi hwekuwedzera zvidzidzo pamusoro pekushanda kwemajini eBjGRF mukunaya kwekusanaya kwemvura uye kupa mageneti ekuberekesa kusanaya kwemvura yemasitadhi.
Makumi matatu nemana emajini eBjGRF akaonekwa muBrassica juncea genome vachishandisa tsvakiridzo mbiri dzeHMMER, dzese dziine QLQ neWRC madomasi. Iwo maCDS akatevedzana emajini eBjGRF akaonekwa anoratidzwa muSupplementary Table S1. BjGRF01–BjGRF34 anopihwa mazita zvichienderana nenzvimbo yavo pachromosome. Iyo physicochemical properties yemhuri iyi inoratidza kuti amino acid urefu hwakasiyana-siyana, kubva ku261 aa (BjGRF19) kusvika 905 aa (BjGRF28). Iyo isoelectric point yeBjGRF inotangira pa6.19 (BjGRF02) kusvika 9.35 (BjGRF03) ine avhareji ye8.33, uye 88.24% yeBjGRF ipuroteni yakakosha. Izvo zvakafanotaurwa molecular weight range yeBjGRF inobva ku29.82 kDa (BjGRF19) kusvika ku102.90 kDa (BjGRF28); iyo instability index yeBjGRF mapuroteni anobva ku51.13 (BjGRF08) kusvika 78.24 (BjGRF19), ose ari makuru kupfuura makumi mana, zvichiratidza kuti fatty acid index inotangira kubva 43.65 (BjGRF01) kusvika 78.78 (BjGRF22), avhareji ye77 hydro. (BjGRF31) kusvika -0.45 (BjGRF22), mapuroteni ose e-hydrophilic BjGRF ane maitiro asina kunaka eGRAVY, izvo zvingave zvichikonzerwa nekushayikwa kwehydrophobicity kunokonzerwa nemasara. Subcellular localization prediction yakaratidza kuti 31 BjGRF encoded mapuroteni anogona kuiswa munharaunda, BjGRF04 inogona kuiswa munzvimbo ye peroxisomes, BjGRF25 inogona kuiswa munzvimbo ye cytoplasm, uye BjGRF28 inogona kuiswa munzvimbo ye chloroplasts (Table 1), zvichiratidza kuti BjGRF04 inogona kuiswa munzvimbo inokosha semupimo wekuita nucleus. transcription factor.
Kuongororwa kwePhylogenetic kwemhuri dzeGRF mumarudzi akasiyana-siyana kunogona kubatsira kudzidza mabasa ejeni. Naizvozvo, iyo yakazara-yakareba amino acid sequences ye35 rapeseed, 16 turnip, 12 mupunga, 10 mapfunde uye 9 Arabidopsis GRFs dzakatorwa uye muti we phylogenetic wakavakwa zvichienderana ne34 yakazivikanwa BjGRF genes (Fig. 1). Mhuri nhatu idzi dzine nhamba dzakasiyana dzenhengo; 116 GRF TFs yakakamurwa kuita mapoka matatu akasiyana (mapoka A~C), ane 59 (50.86%), 34 (29.31%) uye 23 (19.83)% eGRF, zvichiteerana. Pakati pavo, nhengo dzemhuri dzeBjGRF 34 dzakapararira mumhuri nhatu duku: nhengo gumi nenhatu muboka A (38.24%), nhengo gumi nembiri muboka B (35.29%) uye 9 nhengo muboka C (26.47%). Mukuita kwemasitadhi polyploidization, nhamba yeBjGRFs majini mumhuri dzakasiyana dzakasiyana, uye kuwedzera kwemajini uye kurasikirwa kungave kwakaitika. Zvakakosha kuziva kuti hapana kugoverwa kwemupunga nemapfunde GRF muboka C, nepo kune 2 mupunga GRFs uye 1 millet GRF muboka B, uye mazhinji emupunga nemapfunde GRF akaiswa mubazi rimwe chete, zvichiratidza kuti maBjGRF ane hukama hwepedyo nemadicots. Pakati pazvo, zvidzidzo zvakadzika zvakanyanya pamusoro pebasa reGRF muArabidopsis thaliana zvinopa hwaro hwezvidzidzo zvinoshanda zveBjGRFs.
Muti wePhylogenetic wemasitadhi unosanganisira Brassica napus, Brassica napus, mupunga, mapfunde uye nhengo dzemhuri yeArabidopsis thaliana GRF.
Ongororo yeanodzokorora majini mumhuri yemasitadhi GRF. Mutsara we grey kumashure unomiririra chivharo chakaenderana mumasitadhi genome, mutsara mutsvuku unomiririra peya yezvikamu zvakadzokororwa zveBjGRF gene;
BjGRF gene expression pasi pekusanaya kwemvura padanho rechina remashizha. qRT-PCR data inoratidzwa muSupplementary Tafura S5. Kusiyana kwakakura mu data kunoratidzwa nemavara madiki.
Sezvo mamiriro ekunze epasi rose ari kuramba achichinja, kudzidza kuti zvirimwa zvinorarama sei nekusanaya kwemvura uye kuvandudza nzira dzekushivirira kwave kuve nyaya yekutsvagisa inopisa18. Mushure mekusanaya kwemvura, iyo morphological structure, gene expression uye metabolic process yemiti ichachinja, izvo zvinogona kutungamirira kumisa photosynthesis uye metabolic disturbance, inokanganisa goho uye kunaka kwezvirimwa19,20,21. Apo zvinomera zvinonzwa zviratidzo zvekusanaya kwemvura, zvinobudisa vatumwa vechipiri zvakadai seCa2 + uye phosphatidylinositol, kuwedzera intracellular calcium ion concentration uye kumisa hurongwa hwekugadzirisa kweprotein phosphorylation pathway22,23. Mapuroteni ekupedzisira anosanganisirwa zvakananga mukudzivirira kwesero kana kudzora mataurirwo emagetsi emagetsi ane hukama kuburikidza neTFs, achiwedzera kushivirira kwezvirimwa kune kushushikana24,25. Saka, maTF anoita basa rakakosha mukupindura kusanaya kwemvura. Zvinoenderana nekutevedzana uye DNA inosunga zvivakwa zvekusanaya kwemvura inopindura maTF, maTF anogona kukamurwa kuita mhuri dzakasiyana, senge GRF, ERF, MYB, WRKY nedzimwe mhuri26.
Iyo GRF gene mhuri imhando yechirimwa-chaiyo TF inoita mabasa akakosha muzvinhu zvakasiyana-siyana sekukura, kusimukira, kufambisa kwemasaini uye mhinduro dzekudzivirira chirimwa27. Sezvo yekutanga GRF gene yakaonekwa muO. sativa28, yakawanda uye yakawanda GRF genes yakaonekwa mumarudzi akawanda uye inoratidzwa kuti inokanganisa kukura kwemiti, kukura uye kunetseka kupindura8, 29, 30,31,32. Nekuburitswa kweiyo Brassica juncea genome sequence, kuzivikanwa kweBjGRF gene mhuri kwakagoneka33. Muchidzidzo ichi, majini makumi matatu nemana eBjGRF akaonekwa mune yese yemasitadhi genome uye akanzi BjGRF01–BjGRF34 zvichibva pane yavo chromosomal. Ese ane akachengetedzwa zvakanyanya QLQ uye WRC madomasi. Ongororo yezvigadzirwa zve physicochemical yakaratidza kuti misiyano yenhamba dzeamino acid uye zviyero zvemamolekyuli zveBjGRF mapuroteni (kunze kweBjGRF28) hazvina kukosha, zvichiratidza kuti nhengo dzemhuri yeBjGRF dzingave nemabasa akafanana. Gene structure analysis yakaratidza kuti 64.7% yeBjGRF genes ine 4 exons, zvichiratidza kuti BjGRF gene structure inenge yakachengetedzwa mukushanduka, asi nhamba yemaexons muBjGRF10, BjGRF16, BjGRP28 uye BjGRF29 genes yakakura. Zvidzidzo zvakaratidza kuti kuwedzera kana kubviswa kwe exons kana introns kunogona kutungamirira kune mutsauko mukugadzirwa kwemajini uye basa, nekudaro kugadzira majini matsva34,35,36. Nokudaro, isu tinofungidzira kuti intron yeBjGRF yakarasika panguva yekushanduka-shanduka, iyo inogona kukonzera kuchinja kwemajini. Zvichienderana nezvidzidzo zviripo, takaonawo kuti nhamba yemaintrons yakabatana nekutaura kwegene. Kana nhamba ye introns mujene yakakura, geni rinogona kukurumidza kupindura kune zvakasiyana-siyana zvisingafadzi zvinhu.
Kudzokororwa kweGene chinhu chikuru mugenomic uye genetic evolution37. Zvidzidzo zvine hukama zvakaratidza kuti kudzokorora kwemajini hakungowedzere huwandu hweiyo GRF majini, asi inoshandawo senzira yekugadzira majini matsva kubatsira zvirimwa kuti zvienderane neakaipa akasiyana mamiriro ezvakatipoteredza38. Huwandu hwe48 duplicate gene pairs dzakawanikwa muchidzidzo ichi, ese aive ezve dhikirini, zvichiratidza kuti segmental duplications ndiyo nzira huru yekuwedzera huwandu hwemajini mumhuri iyi. Zvave zvichishumwa mumabhuku kuti segmental duplication inogona kusimudzira kukwidziridzwa kweGRF gene nhengo dzemhuri muArabidopsis uye sitiroberi, uye hapana tandem kudzokororwa kweiyi gene mhuri yakawanikwa mune chero yemhando27,39. Mhedzisiro yechidzidzo ichi inopindirana nezvidzidzo zviripo paArabidopsis thaliana nemhuri dzemastrawberry, zvichiratidza kuti mhuri yeGRF inogona kuwedzera huwandu hwemajini uye kugadzira majini matsva kuburikidza nechikamu chechipiri muzvirimwa zvakasiyana.
Muchidzidzo ichi, huwandu hwe34 BjGRF majini akaonekwa mumasitadhi, ayo akakamurwa kuita 3 subfamilies. Aya majini airatidza zvakafanana kuchengetedzwa motifs uye majini zvimiro. Ongororo yeCollinearity yakaratidza 48 peya dzezvikamu zvakadzokororwa mumasitadhi. Iyo BjGRF inosimudzira dunhu ine cis-acting zvinhu zvine chekuita nekupindura kwechiedza, mhinduro yehomoni, mhinduro yekushushikana kwezvakatipoteredza, uye kukura nekusimudzira. Matauriro e34 BjGRF genes akaonekwa padanho rembeu yemasitadhi (midzi, madzinde, mashizha), uye maitiro ekutaura kwe10 BjGRF genes pasi pekusanaya kwemvura. Yakawanikwa kuti maitiro ekutaura eBjGRF genes pasi pekusanaya kwemvura akafanana uye angave akafanana. kubatanidzwa mukusanaya kwemvura Kumanikidza kudzora. BjGRF03 neBjGRF32 majini anogona kuita mabasa akanaka ekudzora mukusanaya kwemvura, nepo BjGRF06 neBjGRF23 vachitamba mabasa mukusanaya kwemvura semiR396 yakananga majini. Pakazere, kudzidza kwedu kunopa hwaro hwehupenyu hwekuwanikwa mune ramangwana reBjGRF gene basa muBrassicaceae zvidyarwa.
Mbeu yemasitadhi yakashandiswa mukuyedza iyi yakapihwa neGuizhou Oil Seed Research Institute, Guizhou Academy of Agricultural Sciences. Sarudza mhodzi dzose wodzidyara muvhu (substrate: ivhu = 3:1), uye unganidza midzi, madzinde nemashizha mushure mechikamu chemashizha mana. Miti yacho yakabatwa ne20% PEG 6000 kuenzanisa kusanaya kwemvura, uye mashizha akaunganidzwa mushure me0, 3, 6, 12 uye 24 maawa. Ese masampuli echirimwa akaomeswa nechando muitrojeni yemvura ndokuzochengetwa mufiriza -80°C kuitira bvunzo inotevera.
Yese data yakawanikwa kana kuongororwa panguva yechidzidzo ichi inosanganisirwa muchinyorwa chakaburitswa uye mafaera ekuwedzera eruzivo.
Nguva yekutumira: Jan-22-2025