Kosakonia oryziphila NP19 semushonga wekusimudzira kukura kwezvirimwa uye mushonga wezvipembenene unouraya zvipembenene mukuderedza kuputika kwemupunga wemhando yeKDML105.

Chidzidzo ichi chinoratidza kuti howa hunokonzerwa nemidzi yeKosakonia oryziphila NP19, hwakabviswa mumidzi yemupunga, mushonga unouraya zvipembenene uye unochengetedza hutachiona hwemupunga, unobatsira kukura kwemiti, uchidzivirira kuputika kwemupunga. Kuedza kwakaitwa muzvimbuzi pamashizha matsva embeu dzemupunga dzinonhuhwirira dzeKhao Dawk Mali 105 (KDML105). Zvakabuda zvakaratidza kuti NP19 yakadzivirira kumera kwemupunga unouraya fungus conidia. Utachiona hwefungus hwakadziviswa pasi pemamiriro matatu akasiyana ekurapa: kubaiwa kwemupunga neNP19 nefungal conidia; kubaiwa kwemupunga neNP19 nefungal conidia panguva imwe chete; uye kubaiwa kwemupunga nefungal conidia zvichiteverwa nekurapwa neNP19 maawa makumi matatu gare gare. Uyezve, NP19 yakaderedza kukura kwefungus hyphal ne9.9–53.4%. Mukuyedza kwemuhari, NP19 yakawedzera mabasa eperoxidase (POD) ne superoxide dismutase (SOD) ne6.1% kusvika 63.0% uye 3.0% kusvika 67.7%, zvichiteerana, zvichiratidza nzira dzakasimba dzekudzivirira zvirimwa. Zvichienzaniswa nedzisina kutapukirwa neNP19, zvirimwa zvemupunga zvine NP19 zvakaratidza kuwedzera kwehuwandu hwepigment ne0.3%–24.7%, huwandu hwezviyo zvakazara pa panicle ne4.1%, goho rezviyo zvakazara ne26.3%, index yehuwandu hwegoho ne34.4%, uye huwandu hwe aromatic compound 2-acetyl-1-pyrroline (2AP) ne10.1%. Muzvirimwa zvemupunga zvine NP19 ne blast, kuwedzera kwacho kwaive 0.2%–49.2%, 4.6%, 9.1%, 54.4%, uye 7.5%, zvichiteerana. Kuedza kwemumunda kwakaratidza kuti zvirimwa zvemupunga zvakatorwa uye/kana kubaiwa neNP19 zvakaratidza kuwedzera kwehuwandu hwezviyo zvakakwana pa panicle ne15.1–27.2%, goho rezviyo zvakakwana ne103.6–119.8%, uye huwandu hwe2AP ne18.0–35.8%. zvirimwa zvemupunga izvi zvakaratidzawo kushanda kwakanyanya kweSOD (6.9–29.5%) zvichienzaniswa nezvirimwa zvemupunga zvine hutachiona hwe blast zvisina kubaiwa neNP19. Kushandiswa kweNP19 mushure mehutachiona kwakaderedza kufambira mberi kwegomarara. Saka, K. oryphila NP19 yakaratidzwa kuva inogona kukura kwezvirimwa zvinokurudzira bioagent uye biopesticide yekudzora blast yemupunga.
Zvisinei, kushanda kwemishonga inouraya fungi kunokonzerwa nezvinhu zvakawanda, zvinosanganisira kugadzirwa kwayo, nguva uye nzira yekushandisa, kuoma kwechirwere, kushanda kwemaitiro ekufanotaura chirwere, uye kubuda kwemarudzi anodzivirira fungi. Uyezve, kushandiswa kwemishonga inouraya fungi kunogona kukonzera chepfu inosara munharaunda uye kuisa njodzi kuhutano hwevashandisi.
Mukuedza kwemuhari, mbeu dzemupunga dzakaiswa mushonga wekuuraya utachiona pamusoro pevhu ndokumera sezvakatsanangurwa pamusoro apa. Dzakazodyarwa neK. oryphila NP19 ndokuiswa mumatireyi embeu. Mbeu dzakaiswa mumatura kwemazuva makumi matatu kuti dzibvumire mbeu dzemupunga kubuda. Mbeu dzakazoiswa mumatura. Munguva yekuisirwa, mbeu dzemupunga dzakaiswa mushonga wekudzivirira hutachiona hwefungus hunokonzera kuputika kwemupunga uye kuyedzwa kuti dzisarambe dzichirwisa.
Mukuedza kwakaitwa mumunda, mbeu dzakamera dzakabatwa neAspergillus oryzae NP19 dzakarapwa nenzira yakatsanangurwa pamusoro apa uye dzakakamurwa kuita mapoka maviri: mbeu dzakabatwa neAspergillus oryzae NP19 (RS) nembeu dzisina hutachiona (US). Mbeu dzakamera dzakadyarwa mumatireyi ane ivhu rakacheneswa (musanganiswa wevhu, makwati emupunga akapiswa, nendove muchiyero che7:2:1 nehuremu) uye dzakachengetwa kwemazuva makumi matatu.
oryziphila conidial suspension yakawedzerwa mumupunga weR uye mushure memaawa makumi matatu ekuisirwa, 2 μl yeK. oryziphila NP19 yakawedzerwa panzvimbo imwe chete. Ndiro dzese dzePetri dzakaisirwa pa25°C murima kwemaawa makumi matatu uye dzakazoisirwa pasi pekuvhenekerwa nguva dzose. Boka rega rega rakadzokororwa katatu. Mushure memaawa makumi manomwe nemaviri ekuisirwa, zvikamu zvemiti zvakaongororwa uye zvakaongororwa ne electron microscopy. Muchidimbu, zvikamu zvemiti zvakaiswa mu phosphate-buffered saline ine 2.5% (v/v) glutaraldehyde uye zvakabviswa mvura mumhinduro dze ethanol dzakatevedzana. Samples dzakaomeswa ne carbon dioxide, ndokuzoiswa gold-coated ndokuonekwa pasi pe scanning electron microscope kwemaminitsi gumi nemashanu.