Howa hukuru hune huwandu hwakawanda uye hwakasiyana hwezvinhu zvinovaka muviri uye hunoonekwa sezvinhu zvinokosha zvehupenyu. Phellinus igniarius howa hukuru hunoshandiswa pakurapa uye pakudya, asi kupatsanurwa kwahwo uye zita rechiLatin zvichiri kukakavadzana. Vachishandisa ongororo ye multigene segment alignment, vaongorori vakasimbisa kuti Phellinus igniarius nemarudzi akafanana ndeerudzi rutsva uye vakagadzira rudzi rweSanghuangporus. Howa hwehoneysuckle Sanghuangporus lonicericola ndeimwe yemhando dzeSanghuangporus dzakazivikanwa pasi rese. Phellinus igniarius yakakwezva kutariswa kukuru nekuda kwehunhu hwayo hwakasiyana hwekurapa, kusanganisira polysaccharides, polyphenols, terpenes, uye flavonoids. Triterpenes ndiwo macompounds akakosha anoshanda mumishonga erudzi urwu, anoratidza antioxidant, antibacterial, uye antibumor activity.
Triterpenoids ine mukana mukuru wekushandiswa mubhizinesi. Nekuda kwekushomeka kwezviwanikwa zveSanghuangporus zvemusango, kuwedzera kushanda kwayo uye goho rayo zvakakosha zvikuru. Pari zvino, kufambira mberi kwakaitwa mukusimudzira kugadzirwa kwemhando dzakasiyana dzemametabolites eSanghuangporus nekushandisa makemikari anokonzera kufashaira mumvura. Semuenzaniso, polyunsaturated fatty acids, fungal elicitors11 uye phytohormones (kusanganisira methyl jasmonate uye salicylic acid14) zvakaratidzwa kuti zvinowedzera kugadzirwa kwetriterpenoid muSanghuangporus. Vanodzora kukura kwezvirimwa(PGRs)inogona kudzora biosynthesis ye second metabolites mumiti. Muchidzidzo ichi, PBZ, murairidzi wekukura kwemiti anoshandiswa zvakanyanya kudzora kukura kwemiti, goho, mhando uye hunhu hwemuviri, akaongororwa. Kunyanya, kushandiswa kwePBZ kunogona kukanganisa nzira ye terpenoid biosynthetic mumiti. Kusanganiswa kwe gibberellins nePBZ kwakawedzera huwandu hwe quinone methide triterpene (QT) muMontevidia floribunda. Kuumbwa kwe terpenoid pathway yemafuta e lavender kwakachinjwa mushure mekurapwa ne 400 ppm PBZ. Zvisinei, hapana mishumo pamusoro pekushandiswa kwePBZ kuhowa.
Pamusoro pezvidzidzo zvinotarisa pakuwedzera kwekugadzirwa kwetriterpene, zvimwe zvidzidzo zvakajekesawo nzira dzekutonga dzetriterpene biosynthesis muMoriformis pasi pesimba remakemikari anokonzera. Parizvino, zvidzidzo zviri kutarisa pakuchinja kwemazinga ekutaura kwemajini echimiro ane chekuita netriterpene biosynthesis munzira yeMVA, izvo zvinotungamira mukuwedzera kwekugadzirwa kweterpenoid.12,14 Zvisinei, nzira dziri pasi pemajini aya anozivikanwa echimiro, kunyanya zvinhu zvinonyora zvinodzora kutaurwa kwawo, hazvisati zvajeka munzira dzekutonga dzetriterpene biosynthesis muMoriformis.
Muchidzidzo ichi, mhedzisiro yehuwandu hwakasiyana hwezvinogadzirisa kukura kwezvirimwa (PGRs) pakugadzirwa kwetriterpene uye kukura kwemycelial panguva yekuvirisa honeysuckle (S. lonicericola) yakaongororwa. Mushure mezvo, metabolomics uye transcriptomics zvakashandiswa kuongorora kuumbwa kwetriterpene uye mapatani ekutaura kwemajini anobatanidzwa mu triterpene biosynthesis panguva yekurapwa kwePBZ. Data reRNA-sequencing uye bioinformatics rakaratidza zvakare chinangwa chekunyora chinyorwa cheMYB (SlMYB). Uyezve, mutants dzakagadzirwa kusimbisa mhedzisiro yekutonga kweSlMYB gene pa triterpene biosynthesis uye kuona potential target majini. Electrophoretic mobility shift assays (EMSA) yakashandiswa kusimbisa kudyidzana kweSlMYB protein nevanokurudzira maSlMYB target majini. Muchidimbu, chinangwa chechidzidzo ichi chaive chekukurudzira triterpene biosynthesis uchishandisa PBZ uye kuona MYB transcription factor (SlMYB) iyo inodzora zvakananga majini e triterpene biosynthetic anosanganisira MVD, IDI, uye FDPS muS. lonicericola mukupindura kuPBZ induction.
Kuburitswa kweIAA nePBZ kwakawedzera zvakanyanya kugadzirwa kwetriterpenoid muhoneysuckle, asi simba rePBZ rekudzikisa raive rakanyanya kuoneka. Saka, PBZ yakawanikwa iriyo inducer yakanakisisa pakuwedzera huwandu hwe100 mg/L, iyo inofanirwa kuongororwa zvakanyanya.
Nguva yekutumira: Nyamavhuvhu-19-2025