Howa hukuru hune hupfumi uye hwakasiyana seti yebioactive metabolites uye hunoonekwa sehunokosha zviwanikwa. Phellinus igniarius ihowa hombe yagara ichishandiswa kune zvese zvekurapa uye zvekudya, asi kupatsanurwa kwayo uye zita rechiLatin rinoramba richinetsa. Vachishandisa multigene segment alignment analysis, vatsvakurudzi vakasimbisa kuti Phellinus igniarius nemamwe marudzi akafanana ndeerudzi rutsva uye akatanga genus Sanghuangporus. Honeysuckle howa Sanghuangporus lonicericola ndeimwe yemhando dzakazivikanwa dzeSanghuangporus pasi rese. Phellinus igniarius yakwezva kutariswa kwakanyanya nekuda kwekusiyana kwayo kwemishonga, kusanganisira polysaccharides, polyphenols, terpenes, uye flavonoids. Triterpenes ndiyo yakakosha pharmacologically inoshanda makomisheni eiyi genus, inoratidzira antioxidant, antibacterial, uye antitumor zviitiko.
Triterpenoids vane mukana mukuru wekutengesa kushandiswa. Nekuda kwekushomeka kwezviwanikwa zvesango zveSanghuangporus mune zvakasikwa, kunyatso kusimudzira kwayo biosynthetic kushanda uye goho kwakakosha kwakakosha. Parizvino, kufambira mberi kwakaitwa mukusimudzira kugadzirwa kweakasiyana echipiri metabolites eSanghuangporus nekushandisa makemikari inducers kudzora akanyudzwa nzira dzekuvirisa. Semuenzaniso, polyunsaturated fatty acids, fungal elicitors11 uye phytohormones (kusanganisira methyl jasmonate uye salicylic acid14) zvakaratidzwa kuwedzera triterpenoid kugadzirwa muSanghuangporus. Plant growth regulators(PGRs)inogona kudzora biosynthesis yechipiri metabolites muzvirimwa. Muchidzidzo ichi, PBZ, chidzoreso chekukura kwechirimwa chinoshandiswa zvakanyanya kudzora kukura kwezvirimwa, goho, kunaka uye hunhu hwemuviri, yakaongororwa. Kunyanya, kushandiswa kwePBZ kunogona kukanganisa terpenoid biosynthetic nzira muzvirimwa. Kusanganiswa kwegibberellins nePBZ kwakawedzera zvinyorwa zvequinone methide triterpene (QT) muMontevidia floribunda.Kuumbwa kweterpenoid nzira yemafuta elavender yakashandurwa mushure mekurapwa ne400 ppm PBZ. Zvakadaro, hapana zvakataurwa pamusoro pekushandiswa kwePBZ kune howa.
Pamusoro pezvidzidzo zvinotarisana nekuwedzera kwekugadzirwa kwetriterpene, zvimwe zvidzidzo zvakare zvakajekesa nzira dzekutonga dze triterpene biosynthesis muMoriformis pasi pesimba remakemikari inducers. Parizvino, zvidzidzo zviri kutarisa kuchinjwa kwemaitiro ekutaura kwemajini ekugadzirisa maitiro ane chokuita ne triterpene biosynthesis mumugwagwa weMVA, iyo inotungamirira kuwedzera kwekugadzirwa kwe terpenoid.12,14 Zvisinei, nzira dziri pasi peiyi mazita emagetsi anozivikanwa, kunyanya zvinyorwa zvinyorwa zvinogadzirisa kutaura kwavo, zvinoramba zvisina kujeka mune triterpenesis biosynthesis.
Muchidzidzo ichi, mhedzisiro yekusiyana kwakasiyana kwezvinomera kukura regulators (PGRs) pakugadzirwa kwetriterpene uye mycelial kukura panguva yekuvirisa yakanyura yehoneysuckle (S. lonicericola) yakaongororwa. Zvadaro, metabolomics uye transcriptomics yakashandiswa kuongorora triterpene kuumbwa uye majini ekutaura maitiro anobatanidzwa mu triterpene biosynthesis panguva yePBZ kurapwa. RNA-kutevedzana uye bioinformatics data yakawedzera kuratidza chinangwa chekunyora chinhu cheMYB (SlMYB). Uyezve, zvinoshanduka zvakagadzirwa kuti zvisimbise mhedzisiro yeSlMYB gene pane triterpene biosynthesis uye kuona zvingangotarirwa majini. Electrophoretic mobility shift assays (EMSA) yakashandiswa kusimbisa kudyidzana kweSlMYB protein nevanokurudzira SlMYB target genes. Muchidimbu, chinangwa chechidzidzo ichi chaive chekukurudzira triterpene biosynthesis uchishandisa PBZ uye kuona MYB transcription factor (SlMYB) inodzora zvakananga triterpene biosynthetic majini anosanganisira MVD, IDI, uye FDPS muS. lonicericola mukupindura PBZ induction.
Induction yezvose zviri zviviri IAA nePBZ yakawedzera zvakanyanya kugadzirwa kwe triterpenoid muhuchi, asi induction mhedzisiro yePBZ yakawedzera kutaurwa. Nokudaro, PBZ yakaonekwa kuti ndiyo yakanakisa inducer pakuwedzera kwe100 mg/L, iyo inofanirwa kuenderera mberi nekudzidza.
Nguva yekutumira: Aug-19-2025