DELLA mapuroteni anochengetedzwa masterkukura regulatorsiyo inoita basa repakati mukudzora kukura kwechirimwa mukupindura mukati uye nharaunda. DELLA inoshanda semugadziri wezvinyorwa uye anonyorerwa kunanga vanosimudzira nekusunga kune zvinyorwa zvinyorwa (TFs) uye histone H2A kuburikidza neiyo GRAS domain. Zvidzidzo zvenguva pfupi yapfuura zvakaratidza kuti kugadzikana kweDELLA kunogadziriswa mushure mekushandura kuburikidza nenzira mbiri: polyubiquitination inokonzerwa ne phytohormone gibberellin, iyo inotungamirira kukurumidza kuparara kwayo, uye kusanganiswa kwediki ubiquitin-like modifiers (SUMO) kuwedzera kuunganidza kwayo. Mukuwedzera, basa reDELLA rinogadziriswa nemaitiro maviri akasiyana glycosylations: DELLA-TF yekudyidzana inowedzerwa neO-fucosylation asi yakavharwa neO-linked N-acetylglucosamine (O-GlcNAc) kugadziriswa. Zvisinei, basa reDELLA phosphorylation rinoramba risingazivikanwi, sezvo zvidzidzo zvekare zvakaratidza migumisiro inopesana, kubva kune iyo inoratidza kuti phosphorylation inokurudzira kana kuderedza DELLA kuderera kune vamwe kuratidza kuti phosphorylation haikanganisi kugadzikana kwayo. Pano, tinoona nzvimbo dzephosphorylation muREPRESSORga1-3(RGA, AtDELLA) yakacheneswa kubva kuArabidopsis thaliana nekuongororwa kwehuwandu hwekutarisa uye kuratidza kuti phosphorylation yemaviri eRGA peptides kumatunhu ePolyS nePolyS/T inosimudzira H2A kusunga uye inovandudza RGA chiitiko. Kudyidzana kweRGA nevanokurudzira vavariro. Zvikuru, phosphorylation haikanganisi RGA-TF kudyidzana kana RGA kugadzikana. Chidzidzo chedu chinoburitsa mamorekuru maitiro ayo phosphorylation induces DELLA chiitiko.
Kuti ujekese basa rephosphorylation mukugadzirisa basa reDELLA, zvakakosha kuona nzvimbo dzeDELLA phosphorylation mu vivo uye kuita ongororo inoshanda muzvirimwa. Nekucheneswa kwekunatswa kwezvidyarwa zvinoteverwa neMS/MS ongororo, takaona akati wandei phosphosites muRGA. Pasi pemamiriro ekushaikwa kweGA, RHA phosphorylation inowedzera, asi phosphorylation haikanganisi kugadzikana kwayo. Zvakakosha, co-IP neChIP-qPCR assays yakaratidza kuti phosphorylation kuPolyS/T nharaunda yeRGA inosimudzira kudyidzana kwayo neH2A uye kushamwaridzana kwayo nevanokurudzira vavariro, ichiburitsa nzira iyo phosphorylation induces RGA basa.
RGA inotorwa kunanga chromatin kuburikidza nekudyidzana kweLHR1 subdomain neTF uye yobva yasunga kuH2A kuburikidza nenharaunda yayo yePolyS/T uye PFYRE subdomain, ichigadzira iyo H2A-RGA-TF yakaoma kudzikamisa RGA. Phosphorylation yePep 2 munharaunda yePolyS/T pakati peDELLA domain uye GRAS domain nekinase isingazivikanwi inosimudzira RGA-H2A kusunga. Iyo rgam2A mutant protein inobvisa RGA phosphorylation uye inotora akasiyana mapuroteni conformation kukanganisa H2A kusunga. Izvi zvinoguma nekudzikamisa kwechinguvana TF-rgam2A kudyidzana uye kupatsanurwa kwe rgam2A kubva kuchinangwa chechromatin. Ichi chifananidzo chinongoratidzira RGA-mediated transcriptional kudzvinyirirwa. Imwe nzira yakafanana inogona kutsanangurwa yeRGA-mediated transcriptional activation, kunze kwekuti iyo H2A-RGA-TF yakaoma yaizosimudzira tarisiro yejini transcription uye dephosphorylation yergam2A inoderedza kunyorwa. Mufananidzo wakagadziridzwa kubva kuHuang et al.21.
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Kuti uwane rumwe ruzivo nezve dhizaini yekudzidza, ona Natural Portfolio Report Abstract ine chekuita nechinyorwa ichi.
Misa spectrometry proteomics data yakapihwa kuProteomeXchange consortium kuburikidza nePRIDE66 shamwari repository ine dataset identifier PXD046004. Mamwe data ese akawanikwa panguva iyi anoratidzwa muSupplementary Information, Supplementary Data Files, uye Raw Data Files. Source data inopihwa pachinyorwa ichi.
Nguva yekutumira: Nov-08-2024