Phosphorylation inomutsa chinodzora kukura kwemasero cheDELLA muArabidopsis nekukurudzira kubatana kwehistone H2A nechromatin.

Mapuroteni eDella akachengetedzwa zvakanyanyavanodzora kukuraizvo zvinoita basa guru mukudzora kukura kwezvirimwa zvichienderana nezviratidzo zvemukati uye zvakatipoteredza. DELLA inoshanda semutongi wekunyora uye inotorwa kuti ishandiswe nevanokurudzira nekubatanidza kune zvinhu zvinonyora (TFs) uye histone H2A kuburikidza nenzvimbo yayo yeGRAS. Zvidzidzo zvechangobva kuitwa zvakaratidza kuti kugadzikana kweDELLA kunodzorwa mushure mekushandurwa kuburikidza nenzira mbiri: polyubiquitination inokonzerwa ne phytohormone gibberellin, iyo inotungamira mukuora kwayo nekukurumidza, uye kubatana kwe small ubiquitin-like modifiers (SUMO) kuti iwedzere kuunganidzwa kwayo. Pamusoro pezvo, basa reDELLA rinodzorwa nenzira inochinja-chinja ne glycosylations mbiri dzakasiyana: kubatana kweDELLA-TF kunowedzerwa neO-fucosylation asi kunodziviswa neO-linked N-acetylglucosamine (O-GlcNAc) modification. Zvisinei, basa reDELLA phosphorylation harisati rajeka, sezvo zvidzidzo zvekare zvakaratidza mhedzisiro inopikisana, kubva kune izvo zvinoratidza kuti phosphorylation inokurudzira kana kuderedza kuora kweDELLA kune dzimwe zvinoratidza kuti phosphorylation haikanganisi kugadzikana kwayo. Pano, tinowana nzvimbo dzephosphorylation muREPRESSORga1-3(RGA, AtDELLA) yakacheneswa kubva kuArabidopsis thaliana nekuongorora kwemass spectrometry uye inoratidza kuti phosphorylation yemapeptide maviri eRGA munzvimbo dzePolyS nePolyS/T inokurudzira kubatana kweH2A uye kuwedzera kweRGA activity. Kubatana kweRGA nevanokurudzira chinangwa. Zvinonyanya kukosha kuti phosphorylation haikanganisi kusangana kweRGA-TF kana kugadzikana kweRGA. Chidzidzo chedu chinoratidza mashandiro emamorekuru ayo phosphorylation inokonzera basa reDELLA.
Kuti tinzwisise basa rephosphorylation mukudzora mashandiro eDELLA, zvakakosha kuziva nzvimbo dzeDELLA phosphorylation mumuviri uye kuita functional analysis mumiti. Nekuchenesa affinity yezvirimwa zvinoteverwa neMS/MS analysis, takaona phosphosite dzakawanda muRGA. Pasi pemamiriro ekushomeka kweGA, RHA phosphorylation inowedzera, asi phosphorylation haikanganisi kugadzikana kwayo. Zvakakosha, co-IP uye ChIP-qPCR assays zvakaratidza kuti phosphorylation munzvimbo yePolyS/T yeRGA inokurudzira kudyidzana kwayo neH2A uye kubatana kwayo nevanokurudzira chinangwa, zvichiratidza nzira iyo phosphorylation inokonzera kushanda kweRGA.
RGA inotorwa kuti iite chinangwa chechromatin kuburikidza nekudyidzana kwechikamu cheLHR1 neTF uye yobva yabatana neH2A kuburikidza nechikamu chayo chePolyS/T uye chikamu chePFYRE, zvichigadzira H2A-RGA-TF complex kuti igare zvakanaka. Phosphorylation yePep 2 munzvimbo yePolyS/T pakati penzvimbo yeDELLA nechikamu cheGRAS nekinase isingazivikanwe inowedzera kubatana kweRGA-H2A. Protein yeRgam2A mutant inobvisa phosphorylation yeRGA uye inotora chimiro chakasiyana cheprotein kuti ipindire kubatana kweH2A. Izvi zvinoguma nekusagadzikana kwekudyidzana kweTF-rgam2A kwenguva pfupi uye kupatsanurwa kweRgam2A kubva kuchromatin yakatarwa. Mufananidzo uyu unoratidza chete kudzvinyirirwa kwetranscriptional kunodzorwa neRGA. Maitiro akafanana anogona kutsanangurwa ekushanda kwetranscriptional kunodzorwa neRGA, kunze kwekuti H2A-RGA-TF complex inosimudzira transcriptional yemajini akatarwa uye dephosphorylation yeRgam2A inodzikisa transcription. Mufananidzo wakagadziridzwa kubva kuna Huang et al.21.
Data rese rehuwandu rakaongororwa nenhamba uchishandisa Excel, uye mutsauko wakakosha wakaongororwa uchishandisa bvunzo yeVadzidzi. Hapana nzira dzehuwandu dzakashandiswa kutanga kuona saizi yemuenzaniso. Hapana data rakabviswa muongororo; kuyedza kwacho hakuna kurongeka; vaongorori havana kuona kugoverwa kwedata panguva yekuedza uye kuongororwa kwemhedzisiro. Saizi yemuenzaniso inoratidzwa munhoroondo yemufananidzo uye faira redata rekwakabva.
Kuti uwane rumwe ruzivo nezve dhizaini yekudzidza, ona Natural Portfolio Report Abstract yakabatana nechinyorwa chino.
Data reMass spectrometry proteomics rakaiswa muProteomeXchange consortium kuburikidza nePRIDE66 partner repository ine dataset identifier PXD046004. Mamwe data ese akawanikwa panguva yekudzidza uku anoratidzwa muSupplementary Information, Supplementary Data Files, uye Raw Data Files. Data resource rakapihwa kuchinyorwa chino.