Vatsvaguriri vanobva kuDhipatimendi reBiochemistry kuIndian Institute of Sciences (IISc) vakawana nzira yagara ichitsvagwa inoshandiswa nemiti yekare yakaita sebryophytes (kusanganisira mosses neliverworts) kutidzora kukura kwezvirimwa- nzira yakachengetedzwawo mumiti inotumbuka maruva munguva pfupi yapfuura.
Chidzidzo ichi, chakaburitswa mumagazini inonzi Nature Chemical Biology, chinotarisa pakudzora kwete kwetsika kwepuroteni yeDELLA, iyo inodzora kukura kwemasero inogona kudzivirira kupatsanurwa kwemasero muzvirimwa zvemudumbu (zvirimwa zvemuvhu).
"DELLA inoita se speed bump, asi kana speed bump iyi ikagara iripo, chirimwa hachigone kufamba," anotsanangura kudaro Debabrata Laha, purofesa we biochemistry uye munyori wekudzidza. Nokudaro, kuora kwemapuroteni eDELLA kwakakosha pakusimudzira kukura kwechirimwa. Mumiti inotumbuka, DELLA inoora kana phytohormone ikaita maruva.gibberellin (GA)Inosunga kune receptor yayo GID1, zvichiita GA-GID1-DELLA complex. Zvadaro, puroteni yeDELLA repressor inosunga kune ubiquitin chains uye inoparadzwa ne26S proteasome.
Zvinofadza kuti, bryophytes dzaive pakati pezvirimwa zvekutanga kugara munyika, makore angangoita mamiriyoni mazana mashanu apfuura. Kunyangwe dzichigadzira phytohormone gibberellin (GA), hadzina GID1 receptor. Izvi zvinomutsa mubvunzo wekuti: kukura nekukura kwezvirimwa izvi zvepakutanga kwaidzorwa sei?
Vaongorori vakashandisa CRISPR-Cas9 system kubvisa gene reVIH rinoenderana, nokudaro vachisimbisa basa reVIH. Zvirimwa zvisina enzyme inoshanda yeVIH zvinoratidza kukura kwakanyanya uye zvikanganiso zvekukura uye kusagadzikana kwemaumbirwo, zvakaita se dense thallus, radial growth impairment, uye kushaya calyx. Kukanganisa uku kwakagadziriswa nekugadzirisa genome yezvirimwa kuti igadzire mugumo mumwe chete (N-terminus) weVIH enzyme. Vachishandisa nzira dzepamusoro dzechromatography, timu yekutsvagisa yakawana kuti N-terminus ine kinase domain inokonzeresa kugadzirwa kweInsP₈.
Vaongorori vakaona kuti DELLA ndeimwe yenzvimbo dzinoshandiswa neVIH kinase mumasero. Uyezve, vakaona kuti chimiro chezvirimwa zvisina MpVIH chakafanana nechezvirimwa zveMiscanthus multiforme zvine kuwedzera kwekutaura kweDELLA.
"Panguva ino, tinoda kuona kana kugadzikana kana kushanda kweDELLA kuri kuwedzera muzvirimwa zvisina MpVIH," akadaro Priyanshi Rana, mudzidzi wedhigirii muboka rekutsvagisa raLahey uye munyori wekutanga wepepa iri. Zvichienderana nefungidziro yavo, vaongorori vakaona kuti kudziviswa kweDELLA kwakadzorera zvakanyanya zvikanganiso zvekukura nekukura muzvirimwa zvakachinja zveMpVIH. Izvi zvakawanikwa zvinoratidza kuti VIH kinase inodzora DELLA zvisina kunaka, nokudaro ichikurudzira kukura nekukura kwezvirimwa.
Vaongorori vakasanganisa nzira dzemajini, dzemakemikari, uye dzezvemuviri kuti vatsanangure mashandiro anoita inositol pyrophosphate kudzora kutaurwa kweprotein yeDELLA mu bryophyte iyi. Kunyanya, InsP₈, inogadzirwa neMpVIH, inobatanidza neprotein yeMpDELLA, ichikurudzira polyubiquitination yayo, izvo zvinozotungamira mukuora kweprotein iyi inodzvinyirira neproteasome.
Tsvagiridzo pamusoro peprotein yeDELLA yakatangira kuGreen Revolution, apo masayendisiti akashandisa mukana wayo wekugadzira mhando dzemhando ...
"Nekukura kwevanhu uye kudzikira kwevhu rinorimwa, kuwedzera kwegoho rezvirimwa kwave kwakakosha," akadaro Raha. Zvichienderana nekuti kudzikira kweDELLA kunodzorwa neInsP₈ kunogona kupararira muzvirimwa zvichangobva kuberekwa, kuwanikwa uku kunogona kugadzira nzira yekukura kwezvirimwa zvechizvarwa chinotevera zvinobereka goho guru.
Nguva yekutumira: Gumiguru-31-2025