Vatsvagiri kubva kuDhipatimendi reBiochemistry kuIndia Institute of Sayenzi (IISc) vakawana nzira yakatsvakwa kwenguva refu inoshandiswa nezvirimwa zvepasi zvekare senge bryophytes (kusanganisira mosses uye liverworts)gadzirisa kukura kwezvirimwa- chigadziriso chakachengetedzwa zvakare mumiti inotumbuka ichangoburwa.
Chidzidzo ichi, chakaburitswa mujenari Nature Chemical Biology, inotarisa pane isiri-yekirasi mutemo weprotein yeDELLA, inyanzvi yekukura inodzora iyo inogona kutadzisa kupatsanurwa kwesero mumiti ye embryonic (zvidyarwa zvevhu).
"DELLA anoita senge bump inomhanya, asi kana bhutsu iyi inogara iripo, chirimwa hachigone kufamba," anotsanangura kudaro Debabrata Laha, purofesa webiochemistry uye munyori pamwe naye wechidzidzo ichi. Naizvozvo, kushatiswa kwemapuroteni eDELLA kwakakosha mukusimudzira kukura kwechirimwa. Mumiti inotumbuka, DELLA inoderedzwa kana iyo phytohormonegibberellin (GA)inosungira kune yayo receptor GID1, ichigadzira iyo GA-GID1-DELLA yakaoma. Zvadaro, iyo DELLA repressor protein inosunga kune ubiquitin cheni uye inoderedzwa ne26S proteasome.
Sezvineiwo, bryophytes yaive pakati pezvirimwa zvekutanga kuita colonize ivhu, angangoita 500 miriyoni makore apfuura. Kunyangwe ivo vachigadzira iyo phytohormone gibberellin (GA), ivo havana iyo GID1 receptor. Izvi zvinomutsa mubvunzo wekuti: kukura nekusimudzirwa kwezvirimwa izvi zvepakutanga zvaidzorwa sei?
Vatsvakurudzi vakashandisa iyo CRISPR-Cas9 system kugogodza iyo inoenderana VIH gene, nokudaro vachisimbisa basa reVIH. Zvirimwa zvisina basa reVIH enzyme inoratidza kukura kwakanyanya uye kuremara kwekukura uye morphological abnormalities, senge dense thallus, kukanganiswa kweradial kukura, uye kushomeka kwecalyx. Izvi zvakakanganisika zvakagadziriswa nekugadzirisa genome yemiti kuti ibudise mugumo mumwe chete (iyo N-terminus) yeVIH enzyme. Ichishandisa advanced chromatography matekiniki, timu yekutsvagisa yakaona kuti N-terminus ine kinase domain inokonzeresa kugadzirwa kweInsP₈.
Vatsvagiri vakawana kuti DELLA ndeimwe yemaserura anotariswa eVIH kinase. Uyezve, vakacherechedza kuti phenotype yeMpVIH-yakashomeka zvidyarwa yakanga yakafanana neyeMiscanthus multiforme zvidyarwa nekuwedzera DELLA kutaura.
"Panguva ino, isu tine shungu dzekuona kana kugadzikana kana chiitiko cheDELLA chichikwidziridzwa muzvirimwa zvinoshomeka zveMpVIH," akadaro Priyanshi Rana, mudzidzi wechiremba muboka rekutsvaga raLahey uye munyori wekutanga webepa. Zvichienderana nefungidziro yavo, vaongorori vakawana kuti DELLA inhibition yakadzoreredza zvakanyanya kukura uye kuremara kwekukura muMpVIH mutant zvidyarwa. Izvi zvakawanikwa zvinoratidza kuti VIH kinase inodzora DELLA zvisina kunaka, nekudaro ichikurudzira kukura nekukura kwechirimwa.
Vatsvakurudzi vakabatanidza genetic, biochemical, uye biophysical nzira kuti vajekese nzira iyo inositol pyrophosphate inogadzirisa DELLA mapuroteni kutaura mune iyi bryophyte. Kunyanya, InsP₈, yakagadzirwa neMpVIH, inosunga kuMpDELLA protein, ichisimudzira polyubiquitination yayo, izvo zvinozotungamira mukuderedzwa kweiyi repressor protein neproteasome.
Tsvagiridzo yeprotein yeDELLA yakatanga kuGreen Revolution, apo masayendisiti nekusaziva akashandisa mukana waainawo kugadzira mhando dzinopa goho rehafu-diki. Kunyange zvazvo maitiro ayo ekuita akanga asingazivikanwe panguva iyoyo, matekinoroji echizvino-zvino akaita kuti masayendisiti ashandise gene editing kushandura basa reprotein iyi, nekudaro vachiwedzera goho rezvirimwa.
"Nekukura kwevanhu uye kuderera kweminda yekurima, kuwedzera goho kwave kunetsa," Raha akadaro. Tichifunga kuti InsP₈-inodzorwa neDELLA degradation inogona kupararira mumiti yeembryo, kuwanikwa uku kunogona kugadzira nzira yekuvandudzwa kwezvirimwa zvinobereka goho guru.
Nguva yekutumira: Oct-31-2025