Uniconazolei triazolemutariri wekukura kwezvirimwaiyo inoshandiswa zvakanyanya kudzora kureba kwemuti uye kudzivirira kukura kwembeu. Zvisinei, maitiro emamorekuru ayo uniconazole inodzivisa kukura kwembeu ye hypocotyl achiri kujeka, uye kune zvidzidzo zvishoma chete zvinosanganisa data re transcriptome ne metabolome kuti zviongorore mashandiro ekukura kwe hypocotyl. Pano, takaona kuti uniconazole yakadzivirira zvakanyanya kukura kwe hypocotyl mumbeu dzemakabheji dzinotumbuka dzeChina. Zvinofadza kuti, zvichibva pakuongorora kwakabatanidzwa kwe transcriptome ne metabolome, takaona kuti uniconazole yakakanganisa zvakanyanya nzira ye "phenylpropanoid biosynthesis". Munzira iyi, jini rimwe chete remhuri ye enzyme regulatory gene, BrPAL4, iyo inobatanidzwa mu lignin biosynthesis, rakaderedzwa zvakanyanya. Pamusoro pezvo, yeast one-hybrid uye two-hybrid assays dzakaratidza kuti BrbZIP39 inogona kubatana zvakananga nenzvimbo ye promoter yeBrPAL4 uye kuita kuti transcription yayo ishande. Sisitimu yekunyaradza majini inokonzerwa nehutachiona yakaratidzawo kuti BrbZIP39 inogona kudzora kurebeswa kwekabheji reChinese nekugadzirwa kwehypocotyl lignin. Zvakabuda muchidzidzo ichi zvinopa ruzivo rutsva pamusoro pemaitiro ekutonga mamorekuru ecloconazole mukudzivirira kurebeswa kwekabheji reChinese nehypocotyl. Zvakasimbiswa kekutanga kuti cloconazole yakaderedza huwandu hwelignin nekudzivirira kugadzirwa kwephenylpropanoid kunosanganisirwa neBrbZIP39-BrPAL4 module, nokudaro zvichikonzera kurebeswa kwekabheji reChinese nehypocotyl.
Kabichi yeChinese (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee) ndeyerudzi rweBrassica uye imuriwo unozivikanwa wegore negore wecruciferous unorimwa zvakanyanya munyika yangu (Wang et al., 2022; Yue et al., 2022). Mumakore achangopfuura, huwandu hwekugadzirwa kwecauliflower yeChinese hwakaramba huchiwedzera, uye nzira yekurima yakachinja kubva pakudzvara zvakananga kuenda pakudzvara zvakanyanya. Zvisinei, mukudzvara zvakanyanya uye kudzvara, kukura kwakanyanya kwehypocotyl kunowanzo buritsa mbesa dzakakora, zvichikonzera kusakwana kwembesa. Naizvozvo, kudzora kukura kwakanyanya kwehypocotyl inyaya huru mukudzvara zvakanyanya uye kudzvara kwekabichi yeChinese. Parizvino, pane zvidzidzo zvishoma zvinosanganisa data re transcriptomics nemetabolomics kuti vaongorore mashandiro ehypocotyl elongation. Maitiro emolecular ayo chlorantazole inodzora kuwedzera kwehypocotyl mukabichi yeChinese haisati yadzidzwa. Chinangwa chedu ndechekuona kuti majini api uye nzira dzemamorekuru dzinopindura ku uniconazole-induced hypocotyl dwarfing muChinese cabbage. Tichishandisa transcriptome uye metabolomic analysis, pamwe ne yeast one-hybrid analysis, dual luciferase assay, uye virus-induced gene silencing (VIGS) assay, takaona kuti uniconazole inogona kukonzera hypocotyl dwarfing muChinese cabbage nekudzivisa lignin biosynthesis muChinese cabbage seeds. Zvakabuda zvedu zvinopa ruzivo rutsva pamusoro pemamorekuru regulatory mechanism iyo uniconazole inodzivisa hypocotyl elongation muChinese cabbage kuburikidza nekudzivisa phenylpropanoid biosynthesis inotungamirirwa neBrbZIP39–BrPAL4 module. Izvi zvinogona kuva nemigumisiro yakakosha pakuvandudza mhando yembeu dzekutengesa uye kubatsira mukuona goho uye mhando yemiriwo.
BrbZIP39 ORF yakazara yakaiswa mu pGreenll 62-SK kuti igadzire effector, uye chidimbu cheBrPAL4 promoter chakasanganiswa ne pGreenll 0800 luciferase (LUC) reporter gene kuti igadzire reporter gene. Mavector e effector nereporter gene akachinjwa kuita mashizha efodya (Nicotiana benthamiana).
Kuti tijekese hukama hwemetabolites nemajini, takaita ongororo yejoint metabolome netranscriptome. Ongororo yeKEGG pathway enrichment yakaratidza kuti maDEG nemaDAM akapfumiswa pamwe chete munzira makumi matatu nematatu dzeKEGG (Mufananidzo 5A). Pakati padzo, nzira ye "phenylpropanoid biosynthesis" ndiyo yakanyanya kupfumiswa; nzira ye "photosynthetic carbon fixation", nzira ye "flavonoid biosynthesis", nzira ye "pentose-glucuronic acid interconversion", nzira ye "tryptophan metabolism", uye nzira ye "starch-sucrose metabolism" zvakapfumiswawo zvakanyanya. Mepu yekupisa (Mufananidzo 5B) yakaratidza kuti maDAM ane chekuita nemaDEG akakamurwa muzvikamu zvakasiyana, pakati pawo maflavonoid aive chikamu chikuru, zvichiratidza kuti nzira ye "phenylpropanoid biosynthesis" yakaita basa rakakosha mu hypocotyl dwarfism.
Vanyori vanoti tsvakiridzo iyi yakaitwa pasina hukama hwezvekutengeserana kana hwemari hunogona kutsanangurwa sekupesana kwezvido.
Pfungwa dzese dzakataurwa muchinyorwa chino ndedzemunyori chete uye hadzisi hadzo dzinoratidza maonero emasangano akabatana, vabudisi, vagadziri, kana vaongorori. Chero zvigadzirwa zvakaongororwa muchinyorwa chino kana zvakataurwa nevagadziri vazvo hazvivimbiswe kana kutsigirwa nemuburitsi.
Nguva yekutumira: Kurume-24-2025