Uniconazoleinonzi triazolechirimwa kukura regulatoriyo inoshandiswa zvakanyanya kudzora kureba kwechirimwa uye kudzivirira kukura kwembesa. Zvisinei, iyo molecular mechanism iyo uniconazole inhibits seedling hypocotyl elongation haisati yanyatsojeka, uye pane zvidzidzo zvishomanana zvinosanganisa data ye transcriptome uye metabolome kuti iongorore nzira ye hypocotyl elongation. Pano, takaona kuti uniconazole yakanyanya kuvharidzira hypocotyl elongation muChinese inotumbuka makabheji madzinde. Sezvineiwo, zvichibva pane yakasanganiswa transcriptome uye metabolome kuongororwa, takaona kuti uniconazole yakakanganisa zvakanyanya "phenylpropanoid biosynthesis" nzira. Munzira iyi, jini rimwe chete reiyo enzyme regulatory gene mhuri, BrPAL4, iyo inobatanidzwa mu lignin biosynthesis, yakaderedzwa zvakanyanya. Pamusoro pezvo, yeast one-hybrid uye maviri-mahybrid assays airatidza kuti BrbZIP39 inogona kusunga zvakananga kudunhu rekusimudzira reBRPAL4 uye kumisa kunyorwa kwayo. Iyo hutachiona-inokonzeresa gene kunyararidza system yakaratidza zvakare kuti BrbZIP39 inogona kudzora zvakanaka hypocotyl kureba kweChinese cabbage uye hypocotyl lignin synthesis. Mhedzisiro yechidzidzo ichi inopa ruzivo rutsva rwema molecular regulatory mechanism ye cloconazole mukudzivisa hypocotyl elongation yeChinese cabbage. Yakasimbiswa kekutanga kuti cloconazole yakaderedza lignin content nekudzivisa phenylpropanoid synthesis inopindirana neBrbZIP39-BrPAL4 module, zvichiita kuti hypocotyl dwarfing muChinese cabbage madzinde.
Chinese cabbage (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee) ndeye genus Brassica uye inonyanyozivikanwa gore negore cruciferous muriwo inorimwa zvakanyanya munyika yangu (Wang et al., 2022; Yue et al., 2022). Mumakore achangopfuura, chiyero chekugadzira cheChinese cauliflower chakaramba chichiwedzera, uye nzira yekurima yachinja kubva pakudyara kwechinyakare kuenda kutsika yakasimba yekudyara uye kudyara. Nekudaro, mukati mekuita tsika yakadzika yembesa uye kuisirwa, yakawandisa hypocotyl kukura kunowanzo kuburitsa mbesa dzemakumbo, zvichikonzera kushata kwembeu. Naizvozvo, kudzora yakawandisa hypocotyl kukura inyaya iri kudzvanya mune yakadzika mbesa tsika uye kutapurirana kweChinese kabichi. Parizvino, pane zvidzidzo zvishomanana zvinobatanidza zvinyorwa zvezvinyorwa uye metabolomics kuti zviongorore maitiro e hypocotyl elongation. Iyo molecular mechanism iyo chlorantazole inodzora hypocotyl kuwedzera muChinese kabichi haisati yadzidzwa. Isu takavavarira kuona kuti ndeapi majini uye mamorekuru nzira dzinopindura kune uniconazole-induced hypocotyl dwarfing muChinese cabbage. Tichishandisa transcriptome uye metabolomic analyses, pamwe neyeast one-hybrid analysis, dual luciferase assay, uye virus-induced gene silencing (VIGS) assay, takaona kuti uniconazole inogona kukonzera hypocotyl dwarfing muChinese cabbage nekudzivisa lignin biosynthesis muChinese cabbage seedling. Mhedzisiro yedu inopa ruzivo rutsva rwemamolecular regulatory mechanism iyo uniconazole inhibits hypocotyl elongation muChinese cabbage kuburikidza nekudzivisa phenylpropanoid biosynthesis inopindirana neBrbZIP39-BrPAL4 module. Mhedzisiro iyi inogona kunge iine zvakakosha zvinoshanda pakuvandudza kunaka kwembeu dzekutengesa uye kubatsira pakuona goho uye kunaka kwemuriwo.
Iyo yakazara-yakareba BrbZIP39 ORF yakaiswa mupGreenll 62-SK kuti igadzire iyo inokonzeresa, uye BrPAL4 chidimbu chekusimudzira chakasanganiswa kune pGreenll 0800 luciferase (LUC) mutori wenhau gene kuti abudise jena renhau. The effector and reporter gene vectors vakashandurwa kuita fodya (Nicotiana benthamiana) mashizha.
Kuti tijekese hukama hwemetabolites uye majini, takaita metabolome yakabatana uye kuongorora kwekunyora. KEGG nzira yekuvandudza ongororo yakaratidza kuti maDEG uye maDAM akabatanidzwa mu33 KEGG nzira (Mufananidzo 5A). Pakati pavo, nzira ye "phenylpropanoid biosynthesis" ndiyo yakanyanya kupfumiswa; iyo "photosynthetic carbon fixation" nzira, iyo "flavonoid biosynthesis" nzira, iyo "pentose-glucuronic acid interconversion" nzira, "tryptophan metabolism" nzira, uye "starch-sucrose metabolism" nzira yakapfumiswawo zvakanyanya. Mepu yekubatanidza kupisa (Mufananidzo 5B) yakaratidza kuti maDAM akabatana neDEGs akakamurwa kuva mapoka akati wandei, pakati peiyo flavonoids yaive chikamu chikuru, zvichiratidza kuti nzira ye "phenylpropanoid biosynthesis" yakaita basa rakakosha mu hypocotyl dwarfism.
Vanyori vanozivisa kuti tsvakiridzo yakaitwa pasina hukama hwekutengesa kana hwemari hunogona kutorwa sekukonana kwekufarira.
Maonero ese anotaurwa muchinyorwa chino ndeemunyori chete uye haafanire kuratidza maonero emasangano akabatana, vatsikisi, vapepeti, kana vaongorori. Chero zvigadzirwa zvakaongororwa muchinyorwa chino kana zvichemo zvakaitwa nevagadziri vazvo hazvina kuvimbiswa kana kutsigirwa nemuparidzi.
Nguva yekutumira: Mar-24-2025