Kudyidzana kwegibberellin nepotassium nitrate, pamwe chete nekupesvedzera kwemupunga pahunhu hwefizikisi yemazambiringa.

Mumazambiringa etafura, kusanganisira echikadzi eSiah-e-Samarkhandi, chimiro chebunch morphology uye saizi yemichero zvakakosha. Zvisinei, kurimwa kwemazambiringa aya kunosangana nematambudziko akati wandei, akadai se berry drop ne dwarf fruit, zvichikonzera kudzikira kwegoho uye kukosha kwemusika. Berry drop ndiyo inonyanya kushungurudza mhando yeSiah-e-Samarkhandi. Nokudaro, chidzidzo ichi chakaongorora mhedzisiro ye 0, 30, 60, uye 90 mg/L⁻¹ GA₃ uye 0 uye 1.5% HKO₃ pakubereka mhando yeSiah-e-Samarkhandi pasi pemamiriro ekubereka akazaruka uye akadzorwa. Pamusoro pezvo, kumwe kuedza kwakaongorora mhedzisiro yezvinotangira mukubereka (Siah-e-Shiraz, Askari, Rotabi, Rishbaba, neAatabaki) pakubereka mhando yeSiah-e-Samarkhandi. Mhedzisiro yacho yakaratidza kuti, kunze kwemhando yeAtabaki, mukubereka kubva kune dzimwe mhando kwakavandudza kubereka kweberry nebunch mumhando yeSiah-e-Samarkhandi. Zvose pamwe chete, musanganiswa we30 mg/Lgibberellin (GA₃)uye 1.5% potassium nitrate (KNO₃) ndiyo yakabatsira zvikuru pakuvandudza kunaka kwemichero nemashizha.
Rudzi urwu rwakakosha zvikuru muIran nedunhu reFars nekuda kwekutsva kwaro uye huwandu hwakawanda hwe anthocyanin. Mazambiringa eSiah-e-Samarkhandi anokura munzvimbo dzisina mvura yakawanda, mvura inonaya kubva pa300 kusvika 450 mm munzvimbo dzakasiyana dzedunhu iri. Nekuti chitarisiko cheboka remazambiringa uye saizi ye berry zvakakosha kuti rive idzva, pane matambudziko akati wandei, akadai sehukuru hwe berry husingawirirani, mhando isina kunaka yeboka, uye huwandu hushoma hwe berry paboka rega rega (nekuda kwekudonha kwemichero), izvo zvinoderedza goho.³ Mbeu yemazambiringa inodyiwa inogona kuva nemigumisiro yakasiyana-siyana yehupenyu, kusanganisira kushanda se antioxidants yechisikigo, zvinochengetedza, uye zvinouraya chikafu, nokudaro zvichidzivirira kusvibiswa kwechikafu netupukanana tunokuvadza.

Kana tichitarisa kuenderana kwemarudzi emazambiringa, marudzi mazhinji anozvishandira ega uye anozvishandira ega. Kuisa manyowa mumiti yakavharwa kwakajairika mumazambiringa. Kunyangwe paine zvimwe zvisingawanzoitiki, hazviwanzoitiki; mamwe marudzi haazvifambise ega. Kubereka kwemichero uye mhando yayo zvinokanganiswa nezvinhu zvakawanda. Chimwe chezvinhu zvakakosha inyaya yekubereka kwemarudzi emazambiringa. Kukura kwakakwana kwenhengo dzemaruva uye kugadzirwa kwemukume wakakodzera une mwero wekumera wakakwira zvakakosha pakuona kuti mukume. Kumera kwemukume kunoenderana nemarudzi, mamiriro ekudya, uye zvinhu zvakatipoteredza, uye mamiriro akanakisa ekumera kwemukume akasiyana.
Kushandiswa kwegibberellin mumazambiringa matsva asina mhodzi kunogona kuwedzera saizi yemichero panguva yekubuda kwemichero. 8.
Zvichienderana nehuwandu hwekurima mazambiringa, kuwana mhinduro dzakakodzera dzekuvandudza kunaka kwawo kwakakosha. Kurapa kwemukume kwakaitwa pamhando dzakadai seSiah-e-Shiraz nedzimwe, sezvo kurapwa uku kwakaguma nekukura kwemukume (data harina kupihwa). Kuisa mukume uyu (mukume une hutano itsime guru reauxin neGA3) pamhando yeSiah-e-Samarkhandi uye kumera kwawo kunokurudzira kukura kwemazai, zvichikonzera kugadzirwa kwemahormone aya akawanda uye, pakupedzisira, kuumbwa kwemichero. Kuvapo kwemukume une hutano mumuchero kunotungamira pakuumbwa kwembeu dzine hutano (Mifananidzo 1A-F). Chinangwa chikuru chekuedza uku kwaive kutsvaga zvikonzero zvekutsemuka kwemichero yemazambiringa uye kushanda kwekurapa kwakadai segibberellin (GA3) uye potassium nitrate (KNO3) uye cross-pollination mukudzivirira kana kuderedza dambudziko iri mumhando yemazambiringa yeSiah-e-Samarkhandi.
Kuedza uku kwakaitwa kwemakore maviri (2021-2022) pamunda wemizambiringa unonaya mvura yekutengeserana mumusha weKhoral, kuchamhembe kwakadziva kumadokero kweShiraz, Iran (35 km kuchamhembe kwakadziva kumadokero kweShiraz, 29°57′ N, 52°14′ S). Dunhu iri rine mamiriro ekunze akapfava, anotonhorera ane avhareji yemvura inonaya pagore ye450 mm uye ivhu revhu rakaita sedope. Minda yemizambiringa yakadyarwa 3.5 metres yakaparadzana mumitsara uye 4 metres pakati peminda yemizambiringa yega yega. Munda wemizambiringa wakanga usingadiridzwi (kurima kunonaya mvura). Kuunganidzwa kwezvirimwa kwaienderana nemirairo nemitemo yakakodzera yemasangano, yenyika, uye yepasi rose uye kwakabvumidzwa nekambani yekurima yezvekurima yekutengeserana yakabatana neShiraz University.
Kuedza kwekutanga nekwechipiri kwakashandisa dhizaini yefactorial yakavakirwa pakugadzira mabhuroko asina kurongeka uye kwakadzokororwa kana.
Kuedza kwechitatu kwaisanganisira kupatsanurana kwembeu (kupatsanurana kwakadzorwa) kwembeu yeSiah-e-Samarghandi uchishandisa mukume kubva mumhando shanu (Rotabi, Rishbaba, Askari, Atabaki, uye Siah-e-Shiraz). Mukume kubva mumbeu yeSiah-e-Samarghandi wakashandiswa pakuzvipatsanura kwembeu iyi uye wakashanda sekutonga mukuedza uku.
Munguva yekutumbuka kwerudzi rwemazambiringa eSiah-e-Samarghandi, mukume wemhando idzi wakaiswa pamaruva mana akasarudzwa. Zuva rimwe kusvika matatu maruva asati atumbuka, maruva akasarudzwa akaiswa mumabhegi epepa. Makumi maviri nemashanu muzana emaruva erudzi rwekutumbuka akaiswa mumabhegi. Mazuva gumi kusvika gumi nemana mushure mekutumbuka, mabhegi ese epepa akabviswa mumainflorescence.
Mushure mekuibva kwemichero (huwandu hwezvinhu zvakaoma zvinonyunguduka ≥16%), goho remazambiringa raiyerwa rimwe nerimwe. Masumbu masere (mana akaiswa mumabhegi, mamwe asina kuiswa mumabhegi) akabva asarudzwa zvisina tsarukano kubva kumativi mana emuzambiringa ndokuendeswa kuchipatara chezvemuviri cheDhipatimendi reHorticulture, Faculty of Agriculture, Shiraz University, Iran, kuti vatsanangure huwandu uye hunhu hwemuzambiringa.
Kuwanda kwemichero kunoverengwa uchishandisa fomura inotevera nekuverenga huwandu hwemaruva mazuva gumi asati atumbuka uye huwandu hwemichero yakaumbwa mazuva gumi mushure mekutumbuka.
Mukuyedza kwekutanga kaviri, michero gumi yakasarudzwa zvisina kurongeka kubva muboka rega rega; mukuyedza kwechitatu, michero makumi mashanu yakasarudzwa. Huwandu hwembeu dziri mubheri rega rega hwakaverengwa, uye huwandu hwembeu pabheri rega rega muboka rega rega rekurapa hwakaverengwa.
Kuti vaone ma phenolic compounds, muchero wemuto wakasanganiswa ne 1:1 ne 80% methanol. Zvadaro, 100 μl ye ethanol extract yakasanganiswa ne 400 μl ye phosphate buffer uye 2.5 ml ye Folin-Ciocalteu reagent (Sigma-Aldrich). Mushure meminiti imwe chete, 2 ml ye 7.5% sodium carbonate solution yakawedzerwa kumusanganiswa, uye sampuli yakaiswa pa 25°C kwemaminitsi mashanu. Kunyudzwa kwakayerwa pa 760 nm uchishandisa spectrophotometer (BioTek Instruments, Inc., USA). Mhedzisiro yacho inoratidzwa se milligrams ye gallic acid pa 100 g yehuremu hutsva, ne gallic acid yakashandiswa.^aschiyero.
Kuwanda kweAnthocyanin kwakaongororwa nenzira ye pH yakasiyana uchishandisa mabuffer maviri akasiyana: 25 mM KCl buffer pa pH 1.0 uye 0.4 M sodium acetate buffer pa pH 4.5. Sampuro yega yega yakaiswa mubuffers dzese kwemaminitsi gumi nemashanu, uye absorbance yakayerwa pa 510 nm ne 700 nm, ne replicates shanu pa sampuro yega yega. Huwandu hwese hweanthocyanin hwakaongororwa zvichienderana nenzira yaSabir et al.
Basa reAntioxidant^{yakanga yakatsunga}uchishandisa nzira ye1,1-diphenyl-2-trinitrophenylhydrazine (DPPH). Nzira chaiyo yaive seinotevera: 100 ml yemuto wemuchero yakasanganiswa nemethanol nemvura nechiyero che1:100. Chidimbu chakazosanganiswa ne2 ml ye 0.1 mM DPPH solution mu methanol. Mushure memaminitsi makumi matatu, kunyudzwa kwemushonga wakabuda kwakayerwa pa517 nm uchishandisa Cecil 2010 UV spectrophotometer. Kunyudzwa kweDPPH pasina chidimbu kwakashandiswa senzira yekudzora. Basa reAntioxidant rakaverengerwa uchishandisa fomura inotevera:
Kuedza uku kwakashandisa dhizaini isina kurongeka zvachose, yakadzokororwa katatu (kudzokorora kwega kwega kwaive nemaboka mana). Data rakaongororwa uchishandisa software yeSAS 9.1, uye bvunzo yaTukey yakashandiswa kuenzanisa nzira padanho rekukosha kwe0.05. Mapombi ekupisa eCluster akagadzirwa uchishandisa software yeR yekuongorora kwakawanda.
Zvichienzaniswa nekurapwa kwekuzviberekesa (14.97%), kukosha kweTSS kwekuberekesa kweAtabaqui kwaive 16.93%, mutsauko mukuru. Hapana mutsauko wakakosha wakaonekwa pakati pemamwe marapirwo nekurapwa kwekuzviberekesa (Mufananidzo 4B).
Kushanda kwakanyanya kweantioxidant kwakaonekwa nekuzviberekesa (55.78%), nepo kushoma kwakaonekwa neatabaca pollen (18.88%) uye askari (31.54%). Mamwe marapirwo haana kusiyana zvakanyanya neboka rekutonga.