Nekubuda kwepagore kwematani anopfuura 700,000, glyphosate ndiyo inonyanya kushandiswa uye yakakura kwazvo mushonga wesora pasi rose.Kuramba masora uye zvinogona kutyisidzira nharaunda uye hutano hwevanhu hunokonzerwa nekushungurudzwa kweglyphosate kwakwezva pfungwa huru.
Musi wa29 Chivabvu, timu yaProfessor Guo Ruiting kubva kuState Key Laboratory yeBiocatalysis uye Enzyme Engineering, yakabatanidzwa pamwe chete neChikoro cheHupenyu Sayenzi yeHubei University nemadhipatimendi epurovhinzi nemakurukota, yakaburitsa bepa rekutsvaga razvino mu Journal of Hazardous Materials, vachiongorora. kuongororwa kwekutanga kwehuswa hwebarnyard.(Iyo yakaipa paddy sora) -yakatorwa aldo-keto reductase AKR4C16 uye AKR4C17 catalyze maitiro ekugadzirisa glyphosate kuora, uye inovandudza zvakanyanya kuparara kweglyphosate neAKR4C17 kuburikidza ne molecular modification.
Kukura glyphosate kuramba.
Kubva payakaunzwa kuma1970, glyphosate yave nemukurumbira pasi rose, uye zvishoma nezvishoma yave mushonga wakachipa, unoshandiswa zvakanyanya uye unonyanya kugadzira mishonga yesora.Inokonzera kukanganisa kwemagetsi mumiti, kusanganisira masora, nekudzivisa zvakananga 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), enzyme inokosha inobatanidzwa mukukura kwemiti uye metabolism.uye rufu.
Naizvozvo, kuuchika mbesa dzeglyphosate-resistant transgenic uye kushandisa glyphosate mumunda inzira yakakosha yekudzora sora mukurima kwemazuva ano.
Nekudaro, nekushandiswa kwakapararira uye kushungurudzwa kweglyphosate, mazhinji emasora akashanduka zvishoma nezvishoma uye akagadzira yakakwira glyphosate kushivirira.
Pamusoro pezvo, glyphosate-resistant genetically modified zvirimwa hazvigone kuora glyphosate, zvichikonzera kuunganidzwa uye kutamiswa kweglyphosate muzvirimwa, izvo zvinogona kupararira nyore nyore kuburikidza nekudya uye kuisa hutano hwevanhu pangozi.
Naizvozvo, zvinokasira kutsvaga majini anogona kushatisa glyphosate, kuitira kurima yakakwira glyphosate-resistant transgenic mbesa ine yakaderera glyphosate masara.
Kugadzirisa chimiro chekristaro uye catalytic reaction maitiro echirimwa-yakatorwa glyphosate-inosvibisa enzymes.
Muna 2019, zvikwata zvekuChina neAustralia zvakaona maviri glyphosate-inosvibisa aldo-keto reductases, AKR4C16 uye AKR4C17, kekutanga kubva kune glyphosate-inodzivirira barnyard huswa.Vanogona kushandisa NADP+ se cofactor kushatisa glyphosate kune nontoxic aminomethylphosphonic acid uye glyoxylic acid.
AKR4C16 uye AKR4C17 ndiwo ekutanga kutaurwa glyphosate-anosvibisa enzymes anogadzirwa nekushanduka-shanduka kwezvirimwa.Kuti vaende mberi vachiongorora mamorekuru ekudzikisira kwavo glyphosate, timu yaGuo Ruiting yakashandisa X-ray crystallography kuongorora hukama pakati peaya ma enzymes uye cofactor yakakwirira.Mamiriro akaomarara echigadziriso akaburitsa nzira yekusunga ye ternary complex yeglyphosate, NADP+ uye AKR4C17, uye yakurudzira iyo catalytic reaction mechanism yeAKR4C16 uye AKR4C17-mediated glyphosate degradation.
Chimiro cheAKR4C17/NADP+/glyphosate yakaoma uye maitiro ekugadzirisa glyphosate degradation.
Molecular modification inovandudza kukanganisa kweglyphosate.
Mushure mekuwana iyo yakanaka-matatu-dimensional structural modhi yeAKR4C17/NADP+/glyphosate, timu yaMuzvinafundo Guo Ruiting yakawedzera kuwana mutant protein AKR4C17F291D ine 70% yekuwedzera mukushatisa kugona kweglyphosate kuburikidza nekuongorora enzyme uye dhizaini ine musoro.
Kuongororwa kweglyphosate-inosvibisa basa reAKR4C17 mutants.
"Basa redu rinoburitsa mamolecular mashandiro eAKR4C16 uye AKR4C17 achikonzeresa kuparara kweglyphosate, iyo inoisa hwaro hwakakosha hwekumwe kugadziridzwa kweAKR4C16 uye AKR4C17 kuvandudza kunaka kwavo kweglyphosate."Anowirirana munyori webepa, Associate Professor Dai Longhai weHubei University akati ivo vakagadzira mutant protein AKR4C17F291D ine yakagadziridzwa glyphosate degradation performance, iyo inopa chishandiso chakakosha pakurima yakakwira glyphosate-inodzivirira transgenic zvirimwa zvine yakaderera glyphosate masara uye kushandisa microbial engineering mabhakitiriya. kuderedza glyphosate munharaunda.
Zvinonzi chikwata chaGuo Ruiting chave kwenguva refu chichiita tsvagiridzo yekuongororwa kwechimiro uye kukurukura kwemaitiro ezve biodegradation enzymes, terpenoid synthases, uye zvinodhaka zvinonangwa mapuroteni ezvinhu zvine chepfu uye zvinokuvadza munzvimbo.Li Hao, muongorori wemubatanidzwa Yang Yu uye mudzidzisi Hu Yumei muchikwata ndivo vanyori vekutanga vebepa, uye Guo Ruiting naDai Longhai ndivo vanyori vanowirirana.
Nguva yekutumira: Jun-02-2022